tagA

UDP-N-acetyl-D-mannosamine transferase

locus: BSU_35750

pI: 10.13

mw: 29.35 kDa

protein length: 256 aa

gene length: 771 bp

function: biosynthesis of teichoic acid

product: UDP-N-acetyl-D-mannosamine transferase

essential: yes

ec: 2.4.1.187

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.4|Biosynthesis of teichoic acid]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.3|Biosynthesis of teichoic acid]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,681,370 3,682,140

Phenotypes of a mutant

• essential [Pubmed|12682299], reduced growth rate, loss of rod-like morphology [Pubmed|19376878]
• inactivation of ''[gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA]'' strongly restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]

The protein

Catalyzed reaction/ biological activity

• N-acetyl-α-D-glucosaminyl-1-diphospho-di-trans,octa-cis-undecaprenol + UDP-N-acetyl-α-D-mannosamine --> H⁺ + N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl di-trans,octa-cis-undecaprenyl diphosphate + UDP (according to UniProt)

Protein family

• glycosyltransferase 26 family (single member, according to UniProt)

Structure

• [PDB|5WB4] (from Thermoanaerobacter italicus, corresponds to aa 35 ... 194, 33% identity)

[SW|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA]-[gene|911A4D957A6C0DFC636A152395119DBFC777DDA2|tagB]
  description: [Pubmed|7952180]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7581998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: repression, [Pubmed|9457886], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]: activation, [Pubmed|12950927], in [regulon|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR regulon]

  regulation

  • repressed when phosphate is available ([protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]) [Pubmed|9457886]
  • the leader mRNA is processed upstream of [gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

References

Reviews

• 24024634

Original publications

• 18156271,9457886,9683503,7952180,7581998,19376878,12950927,22211522,25666134,29794222