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tuaH

sugar transferase

locus: BSU_35540

pI: 8.03

mw: 45.43 kDa

protein length: 397 aa

gene length: 1194 bp

function: biosynthesis of teichuronic acid

product: sugar transferase

essential: no

synonyms: yvhH

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.5|Biosynthesis of teichuronic acid]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.4|Biosynthesis of teichuronic acid]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 3,649,875 3,651,068

The protein

Protein family

• [SW|glycosyltransferase 1 family] (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|5790E3DB39F31017B557C2D3F428C0348A461DA6|tuaA/2]-[gene|4AC72C556ED02A7DE4E1E8D69A0FC926359ACE28|tuaA/1]-[gene|54F96DE549F78E6C1580949A21C84B27AF26C4C6|tuaB]-[gene|873EE7A8C9BBFC6E96B0957B450BA665E3C9222D|tuaC]-[gene|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|tuaD]-[gene|B89FA71C2924CC0690224AFBFA06DB0FF0D97C0A|tuaE]-[gene|6F0FA23892BB1829174F66C464149DB428A8DE18|tuaF]-[gene|6E4F2BA67FF58085D462F6A6FB6497BDF600FCF6|tuaG]-[gene|737B37DA0DA1642AD5C4A824228BFED55FF0652A|tuaH]
  description: [Pubmed|10048024]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10048024], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325,15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|9611818,10627039], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

  regulation

  • expressed during [SW|sporulation] in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|16497325,15699190]

Biological materials

Mutant

• BKE35540 ([gene|737B37DA0DA1642AD5C4A824228BFED55FF0652A|tuaH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTTCATCACCTTTTCTC, downstream forward: _UP4_TAGTCCTATAAATTGGGATG
• BKK35540 ([gene|737B37DA0DA1642AD5C4A824228BFED55FF0652A|tuaH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTTCATCACCTTTTCTC, downstream forward: _UP4_TAGTCCTATAAATTGGGATG

Expression vector

• pGP2178 (expression in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

References

• 16497325,10048024,9611818,10627039