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nifZ
cysteine desulfurase, biosynthesis of 4-thiouridine in tRNA
function
biosynthesis of 4-thiouridine in tRNA
product
cysteine desulfurase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]Gene
Coordinates
3,026,957 3,028,102
The protein
Catalyzed reaction/ biological activity
removes sulfuryl group from cysteine and transfers it to [protein|A64A59C2B15015F13911EC53763D370F780400FD|ThiI] [Pubmed|22773787][sulfur carrier]-H + L-cysteine --> [sulfur carrier]-SH + L-alanine (according to UniProt)Protein family
[SW|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)Paralogous protein(s)
[protein|88ACE6B79534338F7C72F107B35A0B2384007088|SufS], [protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|NifS], [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|YrvO], [protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|YcbU] [SW|Cofactors]
PLPStructure
[PDB|1EG5] (NifS-like protein from Thermotoga maritima, 35% identity, 55% similarity) [Pubmed|10715213]Expression and Regulation
Operons
genes
[gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]-[gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]-[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]
description
[Pubmed|22383849]
view in new tabBiological materials
Mutant
BKE29590 ([gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTTACTCCTTTAT, downstream forward: _UP4_TTAAGGGAAATGATGAGGTABKK29590 ([gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTTACTCCTTTAT, downstream forward: _UP4_TTAAGGGAAATGATGAGGTAReferences
Reviews
Original publications