nifZ [2018-09-12 18:43:25]

cysteine desulfurase, biosynthesis of 4-thiouridine in tRNA

locus: BSU29590

pI: 6.34

mw: 41.33 kDa

protein length: 381 aa

gene length: 1143 bp

function: biosynthesis of 4-thiouridine in tRNA

product: cysteine desulfurase

essential: no

synonyms: iscSB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 3,026,957 → 3,028,102

The protein

Catalyzed reaction/ biological activity

• removes sulfuryl group from cysteine and transfers it to [protein|A64A59C2B15015F13911EC53763D370F780400FD|ThiI] [Pubmed|22773787]

Paralogous protein(s)

• [protein|88ACE6B79534338F7C72F107B35A0B2384007088|SufS], [protein|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|NifS], [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|YrvO], [protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|YcbU]

Structure

• [PDB|1EG5] (NifS-like protein from Thermotoga maritima, 35% identity, 55% similarity) [Pubmed|10715213]

Expression and Regulation

Operons

• genes: [gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]-[gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]-[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE29590 (Δ[gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTTACTCCTTTAT, downstream forward: _UP4_TTAAGGGAAATGATGAGGTA
• BKK29590 (Δ[gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTTACTCCTTTAT, downstream forward: _UP4_TTAAGGGAAATGATGAGGTA

References

Reviews

• 19348578

Original publications

• 10715213,22773787