rpsJ

ribosomal protein

locus: BSU_01150

pI: 10.00

mw: 11.00 kDa

protein length: 102 aa

gene length: 309 bp

function: translation

product: ribosomal protein S10 (BS13)

essential: yes

synonyms: tetA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
• [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]

Gene

Coordinates: 135,364 135,672

Phenotypes of a mutant

• essential [Pubmed|23002217,12682299]

The protein

Protein family

• universal [SW|ribosomal protein] uS10 family (single member, according to UniProt)

Modification

• phosphorylated on Ser-32 during [SW|germination] [Pubmed|26381121]

Structure

• [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]
• [PDB|3R2C] (the [protein|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|NusB]-[protein|751D0B909922D4D931FF3B2285CF142D966FCD48|RpsJ] complex from Aquifex aeolicus) [pubmed|21652641]

[SW|Localization]

• located near the aminoacyl-tRNA site of the 30S ribosomal subunit [Pubmed|23759864]

Expression and Regulation

Operons

• genes: [gene|751D0B909922D4D931FF3B2285CF142D966FCD48|rpsJ]-[gene|FEF94BD8482A41C493A8BEC3278B518E8BB4EDFE|rplC]-[gene|02F0893E814E00187D7B342990838EE5445CA78B|rplD]-[gene|5F3933197343FBBDCBD42B408837F8EEF0A0BEBD|rplW]-[gene|9445AC486A2B0A025030B275C789D227D5694145|rplB]-[gene|5287063586EB218843451386108E45274C7A8D68|rpsS]-[gene|F3DE42C41EF57DC5DFFF0D28129F2CE5DB781D99|rplV]-[gene|E7F4E01A6005C8469EAC64E8DFED96D0F29791A9|rpsC]-[gene|C5CAED1256F082E722EBA0A72647AA043B5681DB|rplP]-[gene|49B6E673E28CF21DF629B90A6AD453C46822BDB0|rpmC]-[gene|657A76B7786DC44861ECAC8FDFC8B4E8B55EF05F|rpsQ]-[gene|2BE91EEB70A1961D3BBE0603648E64E86CD0B708|rplN]-[gene|7E4159B8999B33370B12C18AFE2455507DEF891C|rplX]-[gene|F34C72BF4C3A8FF710D3B65F87324400ECDE3610|rplE]-[gene|0CC562884650F322298BEA28C889E2ABCB01AFDE|rpsN]-[gene|B50A96DCD3E8FB26BBC2819DA11A6E0B040C1C8E|rpsH]-[gene|20485A785D3AF82889F2018D3EA5B5FA33E64E95|rplF]-[gene|B39056862596254F3EC7AB2466B6FB3634621DF0|rplR]-[gene|144D952B05EBBFF41EC92DF906543EA00475FE69|rpsE]-[gene|87741B91E3081B7CC1C8AF275B38A52C066F018C|rpmD]-[gene|BEACD74FE0AE56E8154F3EB8D4247488030BF0D3|rplO]-[gene|5350DC618130E013757EE667B9AFC5F2EC68EC08|secY]-[gene|DD3A39A84E53A9B2ACBDD89DFAE3560E45C08DC1|adk]-[gene|2F2455E189FC61A6B1EC6B66412FC662E44BD3F6|map]-[gene|15EAA4007484D9F82CF4F402FF156165156FDDDE|ybzG]-[gene|BFAF497FB8E871AA84A0864CB08D2059791C7E73|infA]-[gene|F69B475C014087C6DEBE2F50F91862A623D21D1E|rpmJ]-[gene|C8B2EC9E35BDE4FC9B5642B8169F4BD13ACD30AC|rpsM]-[gene|8F83D1839E63016E2FA599A756A161DA5476D4D1|rpsK]-[gene|8B9957188874E9A20D2490730BA216F813D48F36|S56]-[gene|5996A5C25E108A3C4562686BF34A59CB14FD56EB|rpoA]-[gene|0D210B61B8F58DA21D97CFDE441501E31AB50D00|rplQ]
  description: [Pubmed|9371452]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9371452] (two promoters, about 140bp and 200bp upstream of the start codon), in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA [Pubmed|17616982]

  additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of ''rpsJ'' [Pubmed|27120414]

Biological materials

lacZ fusion

• pGP605 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab
• pGP2201 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab
• pGP2439 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab
• pGP2472 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab
• pGP2473 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab
• pGP2828 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab, only hairpin 1
• pGP2829 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab, hairpins 1 and 2
• pGP2830 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab, all three hairpins

References

Reviews

• 15621428

Original publications

• 19653700,9371452,11948165,8635744,23759864,23002217,11007480,26381121,25903689,27120414,28516784,17616982,21652641,30023707