SubtiBank SubtiBank
rpsJ
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rpsJ

ribosomal protein
locus
BSU01150
pI
10.00
mw
11.00 kDa
protein length
102 aa Sequence Blast
gene length
306 bp Sequence Blast
function
translation
product
ribosomal protein S10 (BS13)
essential
yes
synonyms
tetA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]

    • Gene

    Coordinates
    135,364 → 135,672

    Phenotypes of a mutant

  • essential [Pubmed|23002217,12682299]

    • The protein

    Protein family

  • [SW|ribosomal protein] S10P family (according to Swiss-Prot)

    • Modification

  • phosphorylated on Ser-32 during [SW|germination] [Pubmed|26381121]

    • Structure

  • [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]

    • [SW|Localization]

  • located near the aminoacyl-tRNA site of the 30S ribosomal subunit [Pubmed|23759864]

    • Expression and Regulation

    Operons

    genes
    [gene|751D0B909922D4D931FF3B2285CF142D966FCD48|rpsJ]-[gene|FEF94BD8482A41C493A8BEC3278B518E8BB4EDFE|rplC]-[gene|02F0893E814E00187D7B342990838EE5445CA78B|rplD]-[gene|5F3933197343FBBDCBD42B408837F8EEF0A0BEBD|rplW]-[gene|9445AC486A2B0A025030B275C789D227D5694145|rplB]-[gene|5287063586EB218843451386108E45274C7A8D68|rpsS]-[gene|F3DE42C41EF57DC5DFFF0D28129F2CE5DB781D99|rplV]-[gene|E7F4E01A6005C8469EAC64E8DFED96D0F29791A9|rpsC]-[gene|C5CAED1256F082E722EBA0A72647AA043B5681DB|rplP]-[gene|49B6E673E28CF21DF629B90A6AD453C46822BDB0|rpmC]-[gene|657A76B7786DC44861ECAC8FDFC8B4E8B55EF05F|rpsQ]-[gene|2BE91EEB70A1961D3BBE0603648E64E86CD0B708|rplN]-[gene|7E4159B8999B33370B12C18AFE2455507DEF891C|rplX]-[gene|F34C72BF4C3A8FF710D3B65F87324400ECDE3610|rplE]-[gene|0CC562884650F322298BEA28C889E2ABCB01AFDE|rpsN]-[gene|B50A96DCD3E8FB26BBC2819DA11A6E0B040C1C8E|rpsH]-[gene|20485A785D3AF82889F2018D3EA5B5FA33E64E95|rplF]-[gene|B39056862596254F3EC7AB2466B6FB3634621DF0|rplR]-[gene|144D952B05EBBFF41EC92DF906543EA00475FE69|rpsE]-[gene|87741B91E3081B7CC1C8AF275B38A52C066F018C|rpmD]-[gene|BEACD74FE0AE56E8154F3EB8D4247488030BF0D3|rplO]-[gene|5350DC618130E013757EE667B9AFC5F2EC68EC08|secY]-[gene|DD3A39A84E53A9B2ACBDD89DFAE3560E45C08DC1|adk]-[gene|2F2455E189FC61A6B1EC6B66412FC662E44BD3F6|map]-[gene|15EAA4007484D9F82CF4F402FF156165156FDDDE|ybzG]-[gene|BFAF497FB8E871AA84A0864CB08D2059791C7E73|infA]-[gene|F69B475C014087C6DEBE2F50F91862A623D21D1E|rpmJ]-[gene|C8B2EC9E35BDE4FC9B5642B8169F4BD13ACD30AC|rpsM]-[gene|8F83D1839E63016E2FA599A756A161DA5476D4D1|rpsK]-[gene|8B9957188874E9A20D2490730BA216F813D48F36|S56]-[gene|5996A5C25E108A3C4562686BF34A59CB14FD56EB|rpoA]-[gene|0D210B61B8F58DA21D97CFDE441501E31AB50D00|rplQ]
    description
    [Pubmed|9371452]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9371452] (two promoters, about 140bp and 200bp upstream of the start codon), in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

    • regulation

  • strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]

    • additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of ''rpsJ'' [Pubmed|27120414]
    • view in new tab

    Biological materials

    lacZ fusion

  • pGP605 (in [protein|search|pAC6]), available in [SW|Jörg Stülke]'s lab
  • pGP2201 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab
  • pGP2439 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab
  • pGP2472 (in [protein|search|pAC5]), available in [SW|Jörg Stülke]'s lab
  • pGP2473 (in [protein|search|pAC6]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 15621428

    • Original publications

  • 19653700,9371452,11948165,8635744,23759864,23002217,11007480,26381121,25903689,27120414,28516784