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mcsB

locus

BSU_00850

5.07

40.97 kDa

protein length

363 aa Sequence Blast

gene length

1092 bp Sequence Blast

function

control of [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR] activity

product

protein arginine kinase

essential

synonyms

yacI

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]

[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]

[category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

Gene

Coordinates

102,484 103,575

Phenotypes of a mutant

more rapid spore germination as compared to wild type cells [pubmed|31221751]

The protein

Catalyzed reaction/ biological activity

ATP + L-arginyl-[protein] --> ADP + H⁺ + N^ω-phospho-L-arginyl-[protein] (according to UniProt)

Protein family

ATP:guanido phosphotransferase family (single member, according to UniProt)

[SW|Domains]

Phosphagen kinase C-terminal (aa 24-254) (according to UniProt)

Modification

autophosphorylation on specific arginine residues [Pubmed|20852588,21622759], dephosphorylation by [protein|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|YwlE] [Pubmed|20852588,21622759]

Structure

[PDB|6FH1] [pubmed|30962626]

[SW|Localization]

cytoplasm (according to UniProt)

Expression and Regulation

Operons

genes

description

[Pubmed|8793870]

sigma factors

regulatory mechanism

regulation

expressed during germination and spore outgrowth [Pubmed|24244006]

induction during diamide stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|30962353]

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Biological materials

Mutant

MGNA-B930 (yacI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1929 NBRP B. subtilis, Japan]

''mcsB::aphA3'' availbale from the Gerth lab

mcsBC167S::spec available from the Gerth lab

GP1457 (''mcsB''::''aphA3''), available in [SW|Jörg Stülke]'s lab

BP69 (spc), available in [SW|Fabian Commichau]'s lab

BKE00850 ([gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGAATAAAATGCTTTAGCG, downstream forward: _UP4_AAAAGACAGGAGGATGAATC

BKK00850 ([gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGAATAAAATGCTTTAGCG, downstream forward: _UP4_AAAAGACAGGAGGATGAATC

Expression vectors

for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

expression of native ''mcsB'' in ''B. subtilis'': pBP186 (in [SW|pBQ200]), available in [SW|Fabian Commichau]'s lab

Antibody

available in Gerth lab

labs

[SW|Ulf Gerth], Greifswald, Germany

References

Reviews

23375660,19609260,28748186

Original Publications

17380125,16163393,19498169,11179229,9987115,8793870,16497325,19226326,9987115,11544224,20852588,21622759,22517742,24825175,24263382,25610436,26458230,27749819,30962626,31221751,32477307,33101263