SubtiBank SubtiBank
rsfA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

rsfA

probable regulator of transcription of SigF-dependent genes
locus
BSU37620
pI
5.13
mw
29.58 kDa
protein length
258 aa Sequence Blast
gene length
774 bp Sequence Blast
function
control of expression of SigF-dependent genes
product
regulator of SigF activity
essential
no
synonyms
ywfN, ipa-92r

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • Gene

    Coordinates
    3,861,437 → 3,862,213

    Phenotypes of a mutant

  • inactivation of ''[gene|7C0458DB0E3E3AC952FB9DDBC4AD220A3A456359|rsfA]'' reduces sporulation efficiency to 11.6% that of wild type cells [Pubmed|26735940]
  • The protein

    [SW|Localization]

  • forespore (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|7C0458DB0E3E3AC952FB9DDBC4AD220A3A456359|rsfA]
    description
    [Pubmed|7934828]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325,10629188], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|10629188], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]
  • regulatory mechanism

  • [protein|7C0458DB0E3E3AC952FB9DDBC4AD220A3A456359|RsfA]: repression, in [regulon|7C0458DB0E3E3AC952FB9DDBC4AD220A3A456359|RsfA regulon]
  • regulation

  • expressed during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325,15699190,10629188]
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    Biological materials

    Mutant

  • MGNA-A592 (ywfN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/592 NBRP B. subtilis, Japan]
  • 1S132 ( ''rsfA''::''tet''), [Pubmed|10629188], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S132&Search=1S132 BGSC]
  • References

  • 16497325,10629188,7934828,12107147,15699190,26735940