SubtiBank SubtiBank
ymcB

ymcB

tRNA methylthiotransferase
locus
BSU_17010
pI
6.87
mw
58.00 kDa
protein length
509 aa Sequence Blast
gene length
1530 bp Sequence Blast
function
tRNA maturation
product
tRNA methylthiotransferase
essential
no
synonyms
ymcB

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • Gene

    Coordinates
    1,772,843 1,774,372

    The protein

    Catalyzed reaction/ biological activity

  • modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA [Pubmed|20472640]
  • [sulfur carrier]-SH + AH2 + N6-dimethylallyladenosine37 in tRNA + 2 S-adenosyl-L-methionine --> 2-methylsulfanyl-N6-dimethylallyladenosine37 in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H+ + L-methionine + S-adenosyl-L-homocysteine (according to UniProt)
  • Protein family

  • methylthiotransferase family (with [protein|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|YqeV], according to UniProt)
  • Paralogous protein(s)

  • [protein|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|YqeV]
  • [SW|Domains]

  • MTTase N-terminal domain (aa 66-184) (according to UniProt)
  • [SW|TRAM domain] (aa 440-503) (according to UniProt)
  • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]
  • Structure

  • [PDB|4JC0] (from Thermotoga maritima, 33% for aa 70 ... 504) [pubmed|23542644]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]-[gene|EE52DFA35B935E551871D079A9BE877DB2001A3B|ymcA]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • GP1109 (spc), available in [SW|Jörg Stülke]'s lab
  • BKE17010 ([gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATATTTTCTCCTTT, downstream forward: _UP4_GGAGAAGCAATCGAGGTGAA
  • BKK17010 ([gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATATTTTCTCCTTT, downstream forward: _UP4_GGAGAAGCAATCGAGGTGAA
  • References

  • 20472640,23542644