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pncB

nicotinate phosphoribosyltransferase

locus: BSU_31750

pI: 5.09

mw: 56.01 kDa

protein length: 490 aa

gene length: 1473 bp

function: NAD salvage

product: nicotinate phosphoribosyltransferase

essential: yes

ec: 2.4.2.11

synonyms: yueK

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.11|Biosynthesis of NAD(P)]
• [category|SW 2|Metabolism] → [category|SW 2.7|Detoxification reactions]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,259,403 3,260,875

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• 5-phospho-α-D-ribose 1-diphosphate + ATP + H₂O + nicotinate --> ADP + diphosphate + nicotinate β-D-ribonucleotide + phosphate (according to UniProt)

Protein family

• NAPRTase family (single member, according to UniProt)

Structure

• [PDB|2F7F] (from ''Enterococcus faecalis'', 61% identity)

Expression and Regulation

Operons

• genes: [gene|26430DCCEC263E7D03A3BBA4E7D636A250C3208B|pncA]-[gene|7CA7ADDBDDE46B0E6522704FD19C0BCF6FA9E058|pncB]
  description: [Pubmed|22383849]

• genes: [gene|17F2619E74A1604E86ED94E1BEE683D49E437A6C|yueH]-[gene|3918D82756A64E86E96A21ACEB34B0CF992D662D|yueI]-[gene|26430DCCEC263E7D03A3BBA4E7D636A250C3208B|pncA]-[gene|7CA7ADDBDDE46B0E6522704FD19C0BCF6FA9E058|pncB]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B562 (yueK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1561 NBRP B. subtilis, Japan]

References

• 16199587,9274030