SubtiBank SubtiBank
bshB2
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bshB2

N-acetylglucosamine-malate deacetylase, minor enzyme involved in bacillithiol synthesis, may act as amidase for the processing of BSH conjugates
locus
BSU19460
pI
4.51
mw
16.54 kDa
protein length
142 aa Sequence Blast
gene length
426 bp Sequence Blast
function
biosynthesis of bacillithiol
product
N-acetylglucosamin-malate deacetylase
essential
no
synonyms
yojG

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.2|Biosynthesis of bacillithiol]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
  • Gene

    Coordinates
    2,121,641 → 2,122,306

    The protein

    Paralogous protein(s)

  • [protein|60367F0396612C8A6491FD92A73B30BED7215506|BshB1]
  • Expression and Regulation

    Operons

    genes
    [gene|7922A49FBC7CCDC8C585EF6E67D7CC9CC91059F7|yoyC]-[gene|63EC848DB7CC4464B070E65D2C9288851B08BBE3|yojF]-[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]
    description
    (major transcript) [Pubmed|23894131,20308541]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|23894131], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]
  • regulation

  • ''[protein|search|yoyC]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
  • view in new tab

    genes
    [gene|FBF1A8C92149A216D8A46F9E02898D7C5C448E2A|yojE]-[gene|7922A49FBC7CCDC8C585EF6E67D7CC9CC91059F7|yoyC]-[gene|63EC848DB7CC4464B070E65D2C9288851B08BBE3|yojF]-[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]
    description
    [Pubmed|23894131]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulation

  • ''[protein|search|yoyC]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
  • view in new tab

    Biological materials

    Mutant

  • MGNA-B428 (yojG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1427 NBRP B. subtilis, Japan]
  • ''bshB2'' null mutant and ''[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]'' / ''[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]'' double null mutant available in [SW|John Helmann] lab
  • CS212 (''[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]
  • BKE19460 (Δ[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATTATTATTCACCCTT, downstream forward: _UP4_TAATCAAAAAGGTATAAGGC
  • BKK19460 (Δ[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATTATTATTCACCCTT, downstream forward: _UP4_TAATCAAAAAGGTATAAGGC
  • References

    Reviews

  • 28117687
  • Original Publications

  • 20308541,19578333,23894131,23758290,27197833