SubtiBank SubtiBank
xkdH
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

xkdH

PBSX prophage
locus
BSU12620
pI
6.04
mw
13.84 kDa
protein length
118 aa Sequence Blast
gene length
354 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.1|PBSX prophage]
  • Gene

    Coordinates
    1,330,892 → 1,331,248

    The protein

    Structure

  • [PDB|3F3B]
  • Expression and Regulation

    Operons

    genes
    [gene|DD4F52FE386EF75009ECF594ED4E2D1593220E4F|xtmA]-[gene|277F51EB4FE2BAB0FD1808EDE25705E10833AD0A|xtmB]-[gene|E77DD93E3CDCA9B81106E6AB0F18513360AAE9DB|xkdE]-[gene|6542BE447872C1A16C056DA21BD7A23C65F5C543|xkdF]-[gene|38173C76C27A7178CEE66E3642533EB34828C2CB|xkdG]-[gene|81C6812565B3E8ADCB19F52BDFFA9E55B94A2E9D|xkdH]-[gene|5ACCC439AD8849355094F8C5FC6EF29A9077EF58|xkdI]-[gene|6DF19E4546509687CA194F6B44EEB723C9467B9C|xkdJ]-[gene|31B1832E5EAD957EE6A5C7CB0C117EC0F26AA8AB|xkdK]-[gene|683D350D98AC0D27951145AD87830B89C0B4F393|xkdM]
    description
    [Pubmed|2110147]

    sigma factors

  • [protein|458406A4E6824C24493CFC19718F10720AA3B453|Xpf]: sigma factor, [Pubmed|8083174], in [regulon|458406A4E6824C24493CFC19718F10720AA3B453|Xpf regulon]
  • regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
  • view in new tab

    Biological materials

    Mutant

  • BKE12620 (Δ[gene|81C6812565B3E8ADCB19F52BDFFA9E55B94A2E9D|xkdH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTGAATGAGCATCTGCCTGT, downstream forward: _UP4_GTCGCAGTCAGGGATGAAAG
  • BKK12620 (Δ[gene|81C6812565B3E8ADCB19F52BDFFA9E55B94A2E9D|xkdH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTGAATGAGCATCTGCCTGT, downstream forward: _UP4_GTCGCAGTCAGGGATGAAAG
  • References

  • 2110147,8083174