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prpC
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prpC

protein phosphatase
locus
BSU_15760
pI
4.36
mw
27.52 kDa
protein length
254 aa Sequence Blast
gene length
765 bp Sequence Blast
function
antagonist of PrkC-dependent phosphorylation
product
protein phosphatase
essential
no
ec
3.1.3.16
synonyms
yloO
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]

    • Gene

    Coordinates
    1,650,384 1,651,148

    Phenotypes of a mutant

  • A ''prpC'' mutant is less lytic in late stationary phase. [Pubmed|12399479]
  • inactivation of ''[gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]'' reduces sporulation efficiency to 50.7% that of wild type cells [Pubmed|26735940]

    • The protein

    Catalyzed reaction/ biological activity

  • dephosphorylation of [gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|EF-tu]]]-Thr63-P [Pubmed|26056311]
  • H2O + O-phospho-L-seryl-[protein] --> L-seryl-[protein] + phosphate (according to UniProt)
  • H2O + O-phospho-L-threonyl-[protein] --> L-threonyl-[protein] + phosphate (according to UniProt)

    • [SW|Domains]

  • [SW|PPM-type phosphatase domain] (aa 2-243) (according to UniProt)

    • [SW|Cofactors]

  • divalent cations such as magnesium or manganese

    • Effectors of protein activity

  • inhibited by inorganic phosphate and glycero-2-phosphate [Pubmed|16857667]

    • Structure

  • [PDB|1TXO] (from ''Mycobacterium tuberculosis'', 34% identity, 54% similarity) [Pubmed|15530359]

    • Expression and Regulation

    Operons

    genes
    [gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]-[gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]-[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]-[gene|8103B8539338B77CF51B16B7FA76783ED2319F13|rpe]-[gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B133 (yloO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1132 NBRP B. subtilis, Japan]
  • OMG401 (aphA3), available in [SW|Jörg Stülke]'s lab
  • 1A962 (no resistance), [Pubmed|12399479], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A962&Search=1A962 BGSC]
  • 1A964 (no resistance), [Pubmed|12399479], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A964&Search=1A964 BGSC]
  • BKE15760 ([gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTAAGGCTGTTAACAACC, downstream forward: _UP4_CAAGTTGAAGAGGGTGAAGA
  • BKK15760 ([gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTAAGGCTGTTAACAACC, downstream forward: _UP4_CAAGTTGAAGAGGGTGAAGA

    • References

    Reviews

  • 21372323

    • Original publications

  • 19246764,10986276,17693724,12399479,18757537,15530359,23793375,16025310,24390483,25012659,26056311,26735940,27806131