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ywnH

phosphinothricin acetyltransferase

locus: BSU_36560

pI: 5.28

mw: 18.22 kDa

protein length: 163 aa

gene length: 492 bp

function: unknown

product: phosphinothricin acetyltransferase

essential: no

ec: 2.3.1.183

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]

Gene

Coordinates: 3,760,694 3,761,185

The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + phosphinothricin --> CoA + H⁺ + N-acetylphosphinothricin (according to UniProt)

Protein family

• [SW|Acetyltransferase family] (according to UniProt)

[SW|Domains]

• [SW|N-acetyltransferase domain] (aa 1-158) (according to UniProt)

Structure

• [PDB|1VHS] [pubmed|16021622]

Expression and Regulation

Operons

• genes: [gene|891DBC0CAB749A3ED4103CD603C61DED34B058B1|ywnH]-[gene|D09E0307CD931FBAE32B6FF5E19D71A612F0F790|ywnG]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A206 (ywnH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/206 NBRP B. subtilis, Japan]
• BKE36560 ([gene|891DBC0CAB749A3ED4103CD603C61DED34B058B1|ywnH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCATACGTTTTTGGC, downstream forward: _UP4_TCATGATTAGTCTGGATAAA
• BKK36560 ([gene|891DBC0CAB749A3ED4103CD603C61DED34B058B1|ywnH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCATACGTTTTTGGC, downstream forward: _UP4_TCATGATTAGTCTGGATAAA

References

• 27694229,16021622