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pucH
function
purine utilization
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]Gene
Coordinates
3,327,247 3,328,587
The protein
Catalyzed reaction/ biological activity
(S)-allantoin + H2O --> allantoate + H+ (according to UniProt)Protein family
[SW|Metallo-dependent hydrolases superfamily] (according to UniProt)Paralogous protein(s)
[protein|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|PyrC] Structure
[PDB|3HM7] (from ''Bacillus halodurans'', 44% identity, 65% similarity)Expression and Regulation
Operons
genes
[gene|8C53FEBB2BCCE45A686119B7ECE4C266D2C0822A|pucH]
description
[Pubmed|12029039]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]regulation
induced in the presence of purine nucleotides (inducer: allantoin) ([protein|search|PucR]) [Pubmed|12029039]view in new tabBiological materials
Mutant
MGNA-A933 (yunH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/933 NBRP B. subtilis, Japan]BKE32410 ([gene|8C53FEBB2BCCE45A686119B7ECE4C266D2C0822A|pucH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATCCCCCCATGTC, downstream forward: _UP4_TAAGCCCGGCGGATTTTACCBKK32410 ([gene|8C53FEBB2BCCE45A686119B7ECE4C266D2C0822A|pucH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATCCCCCCATGTC, downstream forward: _UP4_TAAGCCCGGCGGATTTTACCReferences