SubtiBank SubtiBank
ssbA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

ssbA

single-strand DNA-binding protein, part of the [SW|replisome], promoter of [protein|search|RecA ]DNA repair center assembly, required to internalize and to recombine ssDNA with homologous resident duplex
locus
BSU40900
pI
4.82
mw
18.60 kDa
protein length
172 aa Sequence Blast
gene length
516 bp Sequence Blast
function
[category|SW 3.1.1|DNA replication], [category|SW 3.1.5|DNA repair/ recombination]
product
single-strand DNA-binding protein
essential
yes
synonyms
ssb

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]
  • Gene

    Coordinates
    4,198,886 → 4,199,404

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • SsbA inhibits ssDNA phosphorylase activity of [protein|64C6D783FF3F41C81B216F798A1DC8071345B1ED|PnpA] [Pubmed|21859751]
  • SsbA co-assembles onto [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB]-coated ssDNA [Pubmed|22373918]
  • important for [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] DNA repair center assembly by recruiting [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO] and [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR] to the [SW|replisome] [Pubmed|24891441]
  • RecA-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] as an accessory factor [Pubmed|25138221]
  • Paralogous protein(s)

  • [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB]
  • Modification

  • phosphorylated on Arg-76 [Pubmed|22517742]
  • phosphorylated on Tyr-82 by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|PtkA], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|PtpZ] [Pubmed|16549871]
  • Effectors of protein activity

  • Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold [Pubmed|16549871]
  • Structure

  • [PDB|3VDY] (SsbB in complex with ssDNA, 63% identity, 87% similarity) [Pubmed|22373918]
  • [SW|Localization]

  • forms foci [Pubmed|21958350]
  • Nucleoid (Mid-cell) [Pubmed|16479537]
  • Expression and Regulation

    Operons

    genes
    [gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]-[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
    description
    [Pubmed|14762004]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|24310371], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • regulation

  • [protein|search|ComK]: transcription activation [Pubmed|11948146]
  • view in new tab

    genes
    [gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
    description
    [Pubmed|24310371,22383849]

    regulation

  • [protein|search|ComK]: transcription activation [Pubmed|11948146]
  • view in new tab

    Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • References

    Reviews

  • 22976189,22933559
  • Original publications

  • 17853894,19730681,11948146,16479537,11948165,16549871,20122408,14762004,20581116,22054219,22373918,22517742,21859751,21170359,21958350,23268446,23536821,23779106,22383849,24310371,24443534,24891441,25138221,26707201,26786319,28575448,28878042,29059392,29059328