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buk
function
utilization of branched-chain keto acids
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]Gene
Coordinates
2,501,549 2,502,640
The protein
Catalyzed reaction/ biological activity
ATP + butanoate --> ADP + butanoyl phosphate (according to UniProt)Protein family
acetokinase family (with [protein|DAA285C86692E8B47D48652E0973AE3FF091CBC3|AckA], according to UniProt)Structure
[PDB|1X9J] (from ''Thermotoga maritima'', 52% identity, 69% similarity)[SW|Localization]
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operons
genes
[gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]
description
[Pubmed|12823818]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]: sigma factor, [Pubmed|10094682], in [regulon|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL regulon]regulatory mechanism
[protein|4CEBD81F485DD0B660E297FFC34A0F5270652184|BkdR]: activation, [Pubmed|10094682], in [regulon|4CEBD81F485DD0B660E297FFC34A0F5270652184|BkdR regulon][protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|10094682], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]regulation
induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]view in new tabBiological materials
Mutant
MGNA-C378 (yqiU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2376 NBRP B. subtilis, Japan]BKE24070 ([gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATAACCTCCAGATCAA, downstream forward: _UP4_TAGACAGACAGGAGTGAGTCBKK24070 ([gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATAACCTCCAGATCAA, downstream forward: _UP4_TAGACAGACAGGAGTGAGTCReferences
12427936,15241682,10094682,12823818