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bioI
function
biosynthesis of biotin
product
cytochrome P450 enzyme
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.1|Biosynthesis/ acquisition of biotin]Gene
Coordinates
3,089,226 3,090,413
The protein
Catalyzed reaction/ biological activity
formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acidsC2-C8-saturated long-chain fatty acyl-[ACP] + 3 O2 + 2 reduced [flavodoxin] --> fatty aldehyde + 3 H+ + 3 H2O + 2 oxidized [flavodoxin] + pimeloyl-[ACP] (according to UniProt)Protein family
[SW|cytochrome P450] family (according to UniProt)Paralogous protein(s)
[protein|A95C28DEE73AD25E28C45B88A7C03C92835F9705|CypA], [protein|D8E72C2523D5E6214C9CBAC2C1B27EA45378E162|YjiB], [protein|EF0F13BB682791E167EFC350BF029B08E8A1F410|PksS] Structure
[PDB|3EJB], [PDB|3EJE] (complex with [protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|AcpA]) [Pubmed|18838690]Expression and Regulation
Operons
genes
[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]
description
[Pubmed|8763940,8892842]
regulatory mechanism
[protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA]: repression, [Pubmed|8763940,8892842], in [regulon|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA regulon]regulation
repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|8763940,8892842]view in new tabBiological materials
Mutant
MGNA-A804 (bioI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/804 NBRP B. subtilis, Japan]BKE30190 ([gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT, downstream forward: _UP4_TAAGCCTAAGAATGTGAGTGBKK30190 ([gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30190 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT, downstream forward: _UP4_TAAGCCTAAGAATGTGAGTGReferences
Reviews
Original publications
18838690,8763940,8892842,15449931,15449930,12538057,20658980,11368323,11472016,14737344,12943422,28196402,28898812