SubtiBank SubtiBank
proI

proI

pyrroline-5-carboxylate reductase
locus
BSU_23800
pI
6.70
mw
30.24 kDa
protein length
278 aa Sequence Blast
gene length
837 bp Sequence Blast
function
biosynthesis of proline
product
pyrroline-5-carboxylate reductase
essential
no
ec
1.5.1.2
synonyms
yqjO

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline]
  • Gene

    Coordinates
    2,473,151 2,473,987

    The protein

    Catalyzed reaction/ biological activity

  • 1-pyrroline-5-carboxylate + NADPH + H+ --> L-proline + NADP+, activity is biologically not relevant [pubmed|28824574]
  • Protein family

  • [SW|pyrroline-5-carboxylate reductase family] [pubmed|28824574]
  • Paralogous protein(s)

  • [protein|542871C77BE841AA59A65FFE8A44AB85BF9180F0|ProH]
  • [SW|Cofactors]

  • NADPH [pubmed|28824574]
  • Structure

  • [PDB|5BSE] (from ''Medicago truncatula'', 33% identity) [pubmed|26579138]
  • Expression and Regulation

    Operons

    genes
    [gene|93000B579F630E9F0C6EED40D6661E5E15141FDE|proI]
    description
    [Pubmed|21784929]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21233158], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|T-box|T-box]: anti-termination, in [regulon|T-box|T-box]
  • regulation

  • induced by proline limitation ([protein|search|T-box]) [Pubmed|21233158]
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    Biological materials

    Mutant

  • MGNA-C395 (yqjO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2393 NBRP B. subtilis, Japan]
  • BKE23800 ([gene|93000B579F630E9F0C6EED40D6661E5E15141FDE|proI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATATCCTCCTTCTAT, downstream forward: _UP4_TAGATGTAAGGACAAAAACA
  • BKK23800 ([gene|93000B579F630E9F0C6EED40D6661E5E15141FDE|proI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGATATCCTCCTTCTAT, downstream forward: _UP4_TAGATGTAAGGACAAAAACA
  • References

    Reviews

  • 25367752
  • Original publications

  • 19258532,21233158,11418582,21784929,28752945,28824574,26579138