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folEB
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folEB

GTP cyclohydrolase IB, required for the initial step of queuosine synthesis ([SW|tRNA modification]), replaces [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|FolE] under conditions of zinc starvation
locus
BSU_03340
pI
7.83
mw
34.63 kDa
protein length
305 aa Sequence Blast
gene length
915 bp Sequence Blast
function
biosynthesis of folate and queuosine
product
zinc-independent GTP cyclohydrolase IB
essential
no
ec
3.5.4.16
synonyms
folE2,yciA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.5|Biosynthesis of folate]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

    • Gene

    Coordinates
    364,259 → 365,173

    The protein

    Catalyzed reaction/ biological activity

  • GTP + H2O --> 7,8-dihydroneopterin 3'-triphosphate + formate + H+ (according to UniProt)

    • Protein family

  • GTP cyclohydrolase IV family (single member, according to UniProt)

    • [SW|Cofactors]

  • activated by a variety of divalent cations [Pubmed|19767425]

    • Structure

  • [PDB|3D1T] [Pubmed|19767425]

    • Expression and Regulation

    Operons

    genes
    [gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]-[gene|456AF184261976FAA73359E81443F7035F457D07|yciB]-[gene|F735802BB483C2C5437240AB2E9FC340EAA5B353|yczL]-[gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]
    description
    [Pubmed|9811636]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12426338], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]: repression, in the presence of zinc [Pubmed|12426338], in [regulon|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur regulon]

    • regulation

  • [SW|folE2]: induced by zinc starvation ([protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]) [Pubmed|12426338]
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    Biological materials

    Mutant

  • BKE03340 (Δ[gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA, downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA
  • BKK03340 (Δ[gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA, downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA

    • References

  • 9811636,12426338,17032654,19767425,27561249