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dgkB
function
biosynthesis of lipoteichoic acid
product
diacylglycerol kinase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.3|Biosynthesis of lipoteichoic acid][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.2|Biosynthesis of lipoteichoic acid]Gene
Coordinates
736,436 737,347
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]The protein
Catalyzed reaction/ biological activity
1,2-diacyl-sn-glycerol + ATP --> 1,2-diacyl-sn-glycero-3-phosphate + ADP + H+ (according to UniProt)Protein family
diacylglycerol/lipid kinase family (with [protein|88AECCE90DC0D6C92C61AB8860A31A830A8DAC42|BmrU] and [protein|ED4C1EA16FF96DA20F12B972CD2458FA3B49DDA5|YtlR], according to UniProt)Paralogous protein(s)
[protein|88AECCE90DC0D6C92C61AB8860A31A830A8DAC42|BmrU] [SW|Domains]
DAGKc domain (aa 1-132) (according to UniProt)Structure
[PDB|3S40] (from ''B. anthracis'', 32% identity, 68% similarity)Expression and Regulation
Operons
genes
[gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]
description
[Pubmed|22383849]
view in new tabgenes
[gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]-[gene|5B5014A68D777DB79179904D5FD3D14DEE0BF1F4|yefC]-[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]-[gene|2E55D086DD5023B98929FB8B61238C00E66B8BCB|yeeB]-[gene|DB1AAC91947A6008F631266E5CC85B58703AFD9D|yeeC]
description
[Pubmed|22383849]
view in new tabBiological materials
Mutant
MGNA-B448 (yerQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1447 NBRP B. subtilis, Japan]GP1391 ''dgkB::cat ltaS::aphA3'', available in [SW|Jörg Stülke]'s labBKE06720 ([gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCTCATCATCCTACT, downstream forward: _UP4_TAAAACTTGGCTTGGTAAGCBKK06720 ([gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCTCATCATCCTACT, downstream forward: _UP4_TAAAACTTGGCTTGGTAAGCReferences
Reviews
Original publications
17535816,22451476,28189581