SAVE THE DATE! 21st International Conference on Bacilli and Gram-Positive Bacteria. Prague, Czech Republic, June 7-11, 2021.

sufA

Fe-S carrier protein

locus: BSU_32160

pI: 4.52

mw: 12.61 kDa

protein length: 120 aa

gene length: 363 bp

function: assembly of Fe-S clusters

product: Fe-S carrier

essential: no

synonyms: yutM

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.3|Biosynthesis of iron-sulfur clusters]

Gene

Coordinates: 3,305,599 3,305,961

The protein

Protein family

• HesB/IscA family (with [protein|5959516D55C9EDBFC46F28C8248D374832895740|YneR], according to UniProt)

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|1R94] (from ''E. coli'') [Pubmed|14705938]

Expression and Regulation

Operons

• genes: [gene|94A3B5EF4ADCFA57A204B584B6D305A62E3E15AE|sufA]
  description: [Pubmed|22383849]

• genes: [gene|185B6596F0468B76BCA85E8753063FAE83445E18|dapF]-[gene|94A3B5EF4ADCFA57A204B584B6D305A62E3E15AE|sufA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A969 (yutM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/969 NBRP B. subtilis, Japan]
• BKE32160 ([gene|94A3B5EF4ADCFA57A204B584B6D305A62E3E15AE|sufA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTATGAACCTCCTTT, downstream forward: _UP4_TAAGCACTAAAAACGGATAG
• BKK32160 ([gene|94A3B5EF4ADCFA57A204B584B6D305A62E3E15AE|sufA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTATGAACCTCCTTT, downstream forward: _UP4_TAAGCACTAAAAACGGATAG

References

• 20097860,14705938