SubtiBank SubtiBank
pabA
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pabA

para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II)
locus
BSU_00750
pI
4.78
mw
21.54 kDa
protein length
194 aa Sequence Blast
gene length
585 bp Sequence Blast
function
biosynthesis of folate and tryptophan
product
para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II) glutamine amidotransferase (subunit B) and anthranilate synthase (subunit II)
essential
no
ec
2.6.1.85
synonyms
trpG,trpX

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.13|Biosynthesis/ acquisition of aromatic amino acids]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.5|Biosynthesis of folate]
  • Gene

    Coordinates
    84,290 84,874

    The protein

    Catalyzed reaction/ biological activity

  • Chorismate + L-glutamine --> anthranilate + pyruvate + L-glutamate (according to UniProt)
  • [SW|Domains]

  • [SW|Glutamine amidotransferase type-1 domain] (aa 1-194) (according to UniProt)
  • Structure

  • [PDB|1I1Q] (from ''Salmonella typhimurium'', 42% identity) [Pubmed|11224570]
  • Expression and Regulation

    Operons

    genes
    [gene|B189AD3E3D18876E1956763182D835A151727392|pabB]-[gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]-[gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC]-[gene|FA9539CB701BC47FD4E849FBA6D7B201B27C6104|sul]-[gene|AAE44B4F73C7C02F7DB3574B6169E0DE46F4B787|folB]-[gene|D2B189C6292ACE1B25658B407C284E9A683EAE5B|folK]-[gene|F373EBC35805DC551E8AFF6FDC7E43D5587C4700|yazB]-[gene|CE45D56BC413555AF4E7914B5307AD967A358452|yacF]-[gene|24CC89DE55B3C19F80B11D106B9FD241174AE3E4|lysS]
    description
    [Pubmed|9084182]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9084182], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulation

  • translation repressed by tryptophan ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB]) [Pubmed|9084182]
  • view in new tab

    Other regulations

  • [protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB]: translation inhibition, [Pubmed|9084182]
  • additional information

  • the [gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]-[gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC] part of the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
  • Biological materials

    Mutant

  • BKE00750 ([gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATCATTTCTCCGC, downstream forward: _UP4_TATCGCAAGGAAGTTATTGC
  • BKK00750 ([gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATCATTTCTCCGC, downstream forward: _UP4_TATCGCAAGGAAGTTATTGC
  • References

    Reviews

  • 16285852,19385727
  • Original Publications

  • 8647825,7515880,9084182,17114263,21815947,11224570