psdR

two-component response regulator ([SW|OmpR family]), induction of [gene|464DD1855E6B3195EE242637612A16C73FF32FC0|psdA]-[gene|AC7F08DC1DF7DD2F9AFF84041D357B286B1244AC|psdB] in response to lipid II-binding lantibiotics (nisin and gallidermin) and internal antimicrobial peptides ([protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC], [protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|SkfA])

locus: BSU_34720

pI: 4.68

mw: 27.37 kDa

protein length: 237 aa

gene length: 714 bp

function: resistance against toxic peptides

product: two-component response regulator ([SW|OmpR family])

essential: no

synonyms: yvcP

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

Gene

Coordinates: 3,567,422 3,568,135

The protein

Protein family

• [SW|OmpR family] of two-component response regulators

[SW|Domains]

• [SW|Response regulatory domain] (aa 3-116) (according to UniProt)

Modification

• phosphorylated by [protein|A9525886E911164BDFF0A55DA9F5FDD1C1B7A3B6|PsdS] on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• [PDB|5DCL] (from Streptococcus agalactiae, 42% identity) [pubmed|26930060]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|985E7F25C809AA5DF372101E64960DF704EED53F|psdR]-[gene|A9525886E911164BDFF0A55DA9F5FDD1C1B7A3B6|psdS]-[gene|464DD1855E6B3195EE242637612A16C73FF32FC0|psdA]-[gene|AC7F08DC1DF7DD2F9AFF84041D357B286B1244AC|psdB]
  description: [Pubmed|21078927]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21078927], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|985E7F25C809AA5DF372101E64960DF704EED53F|PsdR]: activation, [Pubmed|21078927], in [regulon|985E7F25C809AA5DF372101E64960DF704EED53F|PsdR regulon]

  regulation

  • ''[protein|search|psdA]'': induced by nisin, gallidermin ([protein|search|PsdR]) [Pubmed|21078927]

Biological materials

Mutant

• MGNA-B637 (yvcP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1636 NBRP B. subtilis, Japan]
• BKE34720 ([gene|985E7F25C809AA5DF372101E64960DF704EED53F|psdR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTGATCCCCCTTATAT, downstream forward: _UP4_GTCAATCGGAAGGATGAAGC
• BKK34720 ([gene|985E7F25C809AA5DF372101E64960DF704EED53F|psdR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTGATCCCCCTTATAT, downstream forward: _UP4_GTCAATCGGAAGGATGAAGC

References

• 10094672,18394148,14651641,21283517,23504016,21078927,26364265,27188294,27997719,26930060