SubtiBank SubtiBank
bacF
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bacF

aminotransferase involved in bacilysin synthesis
locus
BSU_37690
pI
5.35
mw
44.54 kDa
protein length
399 aa Sequence Blast
gene length
1200 bp Sequence Blast
function
biosynthesis of the antibiotic bacilysin
product
pyridoxal 5-phosphate-dependent stereospecific aminotransferase
essential
no
ec
2.6.1.1
synonyms
ywfG,ipa-85d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

    • Gene

    Coordinates
    3,868,287 3,869,486

    The protein

    Catalyzed reaction/ biological activity

  • YwfG catalyzes transamination of tetrahydro-4-hydroxyphenylpyruvate (with L-Phe as amino donor), to form tetrahydrotyrosine [Pubmed|20052993]

    • Protein family

  • [SW|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|938E08D2C50649A52EC06B9A08C6A73598E68CE1|MtnE]

    • [SW|Cofactors]

  • PLP

    • Structure

  • [PDB|6L1L] (apo-protein) [pubmed|32134000]
  • [PDB|6L1N] (substrate-bound) [pubmed|32134000]
  • [PDB|6L1O] (product-bound) [pubmed|32134000]
  • [PDB|2O1B] (from Staphylococcus aureus, 40% identity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|3F49F4AEFC49C2883EFA60FEB3E77591F184195B|bacA]-[gene|0EEE23875DB720D256D53F00D1AD5EF24B538B27|bacB]-[gene|20A7DBA142F3BF8DCAD9732BD908CE8CC79AA993|bacC]-[gene|2CE0C0484A70EEE40277996CD3D564A84FEAF5B3|bacD]-[gene|4D2AD84E74888EC61E44DE5B8E31812F43250B05|bacE]-[gene|99434727013FCCD6E570AE550560A8546F7F2A41|bacF]
    description
    [Pubmed|12372825]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|19801406], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12372825,21709425], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|12697329,21709425], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|19801406], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]

    • regulation

  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A514 (ywfG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/514 NBRP B. subtilis, Japan]
  • BKE37690 ([gene|99434727013FCCD6E570AE550560A8546F7F2A41|bacF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACATCGGACGGTGTTATTT, downstream forward: _UP4_TCCCGCTAAAAAGCGGGATG
  • BKK37690 ([gene|99434727013FCCD6E570AE550560A8546F7F2A41|bacF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACATCGGACGGTGTTATTT, downstream forward: _UP4_TCCCGCTAAAAAGCGGGATG

    • References

  • 12372825,19801406,20052993,21948839,12697329,21709425,32134000