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ykkE
formyltetrahydrofolate deformylase
function
purine nucleotide synthesis
product
formyltetrahydrofolate deformylase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]Gene
Coordinates
1,377,243 1,378,145
The protein
Catalyzed reaction/ biological activity
(6S)-10-formyltetrahydrofolate + H2O --> (6S)-5,6,7,8-tetrahydrofolate + formate + H+ (according to UniProt)Protein family
PurU family (single member, according to UniProt)Paralogous protein(s)
[protein|7B30F27D6C459437967A64467E52961F4A9E0F2C|PurN] [SW|Domains]
[SW|ACT domain] (aa 21-102) (according to UniProt)Structure
[PDB|3W7B] (from ''Thermus thermophilus'', 58% identity) [Pubmed|24108189]Expression and Regulation
Operons
genes
[gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]
description
[Pubmed|22383849]
view in new tabBiological materials
Mutant
MGNA-B310 (ykkE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1309 NBRP B. subtilis, Japan]BKE13110 ([gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATAATCCCTCTTATC, downstream forward: _UP4_TAGACTGCAAGAGGCCCGCGBKK13110 ([gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATAATCCCTCTTATC, downstream forward: _UP4_TAGACTGCAAGAGGCCCGCGReferences