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ykkE

formyltetrahydrofolate deformylase

locus: BSU_13110

pI: 5.95

mw: 34.34 kDa

protein length: 300 aa

gene length: 903 bp

function: purine nucleotide synthesis

product: formyltetrahydrofolate deformylase

essential: no

ec: 3.5.1.10

synonyms: purU

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]

Gene

Coordinates: 1,377,243 1,378,145

The protein

Catalyzed reaction/ biological activity

• (6S)-10-formyltetrahydrofolate + H₂O --> (6S)-5,6,7,8-tetrahydrofolate + formate + H⁺ (according to UniProt)

Protein family

• PurU family (single member, according to UniProt)

Paralogous protein(s)

• [protein|7B30F27D6C459437967A64467E52961F4A9E0F2C|PurN]

[SW|Domains]

• [SW|ACT domain] (aa 21-102) (according to UniProt)

Structure

• [PDB|3W7B] (from ''Thermus thermophilus'', 58% identity) [Pubmed|24108189]

Expression and Regulation

Operons

• genes: [gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B310 (ykkE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1309 NBRP B. subtilis, Japan]
• BKE13110 ([gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATAATCCCTCTTATC, downstream forward: _UP4_TAGACTGCAAGAGGCCCGCG
• BKK13110 ([gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATAATCCCTCTTATC, downstream forward: _UP4_TAGACTGCAAGAGGCCCGCG

References

• 24108189,22383849