ktrB [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

high affinity potassium transporter KtrA-KtrB, integral membrane subunit

locus: BSU31100

pI: 10.00

mw: 48.00 kDa

function: potassium uptake

product: high affinity potassium transporter KtrA-KtrB,
integral membrane subunit (proton symport)

essential: no

synonyms: yubG

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|coping with hyper-osmotic stress], [SW|membrane proteins]

This gene is a member of the following [SW|regulons]

• [SW|YdaO riboswitch]

Gene

Coordinates on the chromosome (coding sequence): 3,189,089 -> 3,190,426

The protein

Paralogous protein(s)

• [protein|search|KtrD]

Structure

• [PDB|4J7C] (the [protein|search|KtrA]-[protein|search|KtrB] complex) [Pubmed|23598340]

[SW|Localization]

• integral membrane protein [Pubmed|12562800]

[SW|Interactions]

• KtrB forms dimers [Pubmed|17932047]
• [protein|search|KtrA]-[protein|search|KtrB] [Pubmed|12562800], composed of an octameric [protein|search|KtrA] ring and two [protein|search|KtrB] dimers [Pubmed|23598340]

Expression and Regulation

Operon

• ''[protein|search|ktrA]-[protein|search|ktrB]'' [Pubmed|12562800]

Regulatory mechanism

• expression is controlled via termination antitermination by the [SW|ydaO riboswitch], expression is switched off upon binding od c-di-AMP [Pubmed|24141192,20511502]

Biological materials

Mutant

• MGNA-A227 (yubG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/227 NBRP B. subtilis, Japan]
• 1A954 ( ''ktrB''::''kan''), [Pubmed|12562800], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A954&Search=1A954 BGSC]
• GHB1 (D(''[protein|search|ktrA]-[protein|search|ktrB]'')::''aphA3''), available in [SW|Erhard Bremer]'s lab
• GP92 (D(''[protein|search|ktrA]-[protein|search|ktrB]'')::''aphA3''), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

Reviews

• 25869574,21680052

Original publications

• 12562800,20511502,17932047,23086297,23598340,16990138,24141192,26771197