SubtiBank SubtiBank
recA
The second international online conference #Subtillery2021 will be held 14th - 18th June - save the date, for more information see the conference website!

The 21st International Conference on Bacilli has been postponed to 2022 and will take place in Prague.

recA

multifunctional protein involved in homologous recombination and DNA repair ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]-autocleavage), required to internalize and to recombine ssDNA with homologous resident duplex, required for efficient survival and replication restart after replication-transcription conflicts
locus
BSU_16940
pI
4.88
mw
37.93 kDa
protein length
348 aa Sequence Blast
gene length
1047 bp Sequence Blast
function
DNA repair/ recombination
product
multifunctional protein involved in homologous recombination and DNA repair ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]-autocleavage)
essential
no
synonyms
recE

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • Gene

    Coordinates
    1,764,645 1,765,691

    Phenotypes of a mutant

  • slower growth [Pubmed|26930481]
  • drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]
  • reduced [SW|sporulation] efficiency [Pubmed|26930481]
  • strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
  • no amplification of the [gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB] chromosomal region to suppress the glutamate auxotrophy of a [gene|87BCAE725B02860156D50E1783F6DB68510C811E|gltC] mutant [pubmed|28294562]
  • sensitive to Cr(VI) treatment [pubmed|30745368]
  • reduced resistance towards electron beams [pubmed|31948638]
  • reduced viability of a [gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA] [gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] double mutant [pubmed|32117122]
  • suppression of lethality of [gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA] inactivation [pubmed|32793628]
  • The protein

    Catalyzed reaction/ biological activity

  • RecA stimulates ssDNA phosphorylase activity of [protein|64C6D783FF3F41C81B216F798A1DC8071345B1ED|PnpA] [Pubmed|21859751]
  • RecA-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] as an accessory factor [Pubmed|25138221]
  • RecA-dATP catalyzes strand exchange even in the absence of the accessory factors [Pubmed|25138221]
  • protects sporulating cells from DNA damage [Pubmed|26930481]
  • contributes to transfection with naked phage DNA [pubmed|31876108]
  • [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] polymerized on tailed SPP1 duplex intermediates invades a homologous region in another incomplete molecule, and in concert with [protein|A3D05FE662CCCFE79B3CB38486206413B84E5D80|RecD2] helicase, reconstitutes a complete linear phage genome with redundant regions at the ends of the molecule [pubmed|31876108]
  • Protein family

  • RecA family (together with [protein|151F226370D225776F3FE7EA4901485095F1AC45|RadA]) (according to UniProt)
  • Modification

  • phosphorylated on Arg-58 [Pubmed|22517742]
  • phosphorylated on Ser-2 [Pubmed|20509597] by [protein|E86A96B832351FF513DD9853EAD8998CC44C9951|YabT] [Pubmed|23634894]
  • Effectors of protein activity

  • [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO] and [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA] provide [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] access to ssDNA during chromosomal transformation [Pubmed|22373918]
  • interaction with [protein|2B091CCEE9E34D659771E39B1FC9050A145048AB|DisA] inhibits [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] filament growth and [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA]-mediated DNA strand exchange [pubmed|30916351]
  • Structure

  • [PDB|1UBC] (RecA from ''Mycobacterium smegmatis'', 67% identity) [pubmed|12837805]
  • [SW|Localization]

  • colocalizes to the [SW|replisome] in response to endogenous and exogenous DNA damage and in response to damage-independent fork arrest (formation of DNA repair centers), repair center formation depends on [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO] and [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR], and is facilitated by [protein|EBE03AEC7EC594A3115A7A72194BDFF300AA0BFA|RecF] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] [Pubmed|24891441]
  • Nucleoid (Mid-cell) [Pubmed|16479537]
  • localizes to one cell pole [Pubmed|21278288]
  • co-localizes with the DNA uptake machinery [Pubmed|17630974]
  • forms a transient, mobile focus associated with the chromosome during spore development [Pubmed|23634894]
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]
    description
    [Pubmed|7690748]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7690748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|8226626,11555642,16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]
  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|7690748], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • regulation

  • induced by conditions that trigger development of genetic competence ([protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]) [Pubmed|7690748]
  • expression is induced in the presence of Cr(VI) [pubmed|30745368]
  • view in new tab

    Biological materials

    Mutant

  • IRN444 (cat), available in [SW|Jörg Stülke]'s lab
  • GP2542(Δ[gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]::spc trpC2), available in [SW|Jörg Stülke]'s lab
  • 1A746 (Δ''recA''::''erm''), [Pubmed|1391055], available at the [http://bgsc.org/getdetail.php?bgscid=1A746 Bacillus Genetic Stock Center]
  • 1A786 (Δ''recA''::''kan''), [Pubmed|11208805], available at the [http://bgsc.org/getdetail.php?bgscid=1A786 Bacillus Genetic Stock Center]
  • BP469 (Δ''recA''::''erm''), available in [SW|Fabian Commichau]'s lab
  • BKE16940 (''[gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]''::''erm'', available in the [http://bgsc.org/ Bacillus Genetic Stock Center], in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s labs) [pubmed|28189581]
  • BKE16940 ([gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]::erm [gene|search|trpC2]) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTATTTTTTCCTCCTT, downstream forward: _UP4_TAAAAATAAAATAAGTTTCA
  • BKK16940 ([gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTATTTTTTCCTCCTT, downstream forward: _UP4_TAAAAATAAAATAAGTTTCA
  • Expression vectors

  • for expression, purification in ''E. coli'' with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • labs

  • [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
  • References

    Reviews

  • 14527291,12045091,10506835,14616075,21517913,23046409,23380520,22933559,17364684,26459995,31950915,32286623
  • Original publications

  • 11814663,16061691,19060143,17803906,16024744,17630974,8226626,11555642,16479537,19730681,7690748,17229847,16267290,20509597,20723756,17449621,21278288,22373918,22517742,23284295,23536821,21859751,23634894,23779106,24285298,24285298,24373815,15378759,24362571,8899710,24670664,24891441,25138221,25169108,25939832,26001966,26786319,26930481,28294562,28344191,28911099,30050509,30254116,30401797,30745368,30814990,30877841,12837805,30975899,30916351,31350886,31876108,31948638,32117122,32793628