SubtiBank SubtiBank
thiI
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thiI

sulfuryl transferase, biosynthesis of 4-thiouridine in tRNA
locus
BSU_29580
pI
9.66
mw
40.17 kDa
protein length
401 aa Sequence Blast
gene length
1206 bp Sequence Blast
function
biosynthesis of 4-thiouridine in tRNA
product
sulfuryl transferase
essential
no
synonyms
ytbJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

    • Gene

    Coordinates
    3,025,748 3,026,953

    The protein

    Catalyzed reaction/ biological activity

  • receives a sulfuryl group from [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|NifZ] and transfers it to uridine at position 8 of tRNA [Pubmed|22773787]
  • [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uridine in tRNA + ATP + H+ + 2 reduced [2Fe-2S]-[ferredoxin] --> [ThiI sulfur-carrier protein]-L-cysteine + 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
  • [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] --> [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H+ + L-cysteinyl-[cysteine desulfurase] (according to UniProt)

    • Protein family

  • thiI family (single member, according to UniProt)

    • [SW|Domains]

  • THUMP domain (aa 60-165) (according to UniProt)

    • Structure

  • [PDB|4KR6] (ThiI-RNA complex from Thermotoga maritima) [pubmed|24705700]
  • [PDB|2C5S] (from B. anthracis) [pubmed|16343540]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]-[gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]-[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • BKE29580 ([gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCT
  • BKK29580 ([gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCT

    • References

  • 14567704,22773787,16343540,24705700