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thiI
sulfuryl transferase, biosynthesis of 4-thiouridine in tRNA
function
biosynthesis of 4-thiouridine in tRNA
product
sulfuryl transferase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]Gene
Coordinates
3,025,748 3,026,953
The protein
Catalyzed reaction/ biological activity
receives a sulfuryl group from [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|NifZ] and transfers it to uridine at position 8 of tRNA [Pubmed|22773787][ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uridine in tRNA + ATP + H+ + 2 reduced [2Fe-2S]-[ferredoxin] --> [ThiI sulfur-carrier protein]-L-cysteine + 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] --> [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H+ + L-cysteinyl-[cysteine desulfurase] (according to UniProt)Protein family
thiI family (single member, according to UniProt)[SW|Domains]
THUMP domain (aa 60-165) (according to UniProt)Structure
[PDB|4KR6] (ThiI-RNA complex from Thermotoga maritima) [pubmed|24705700][PDB|2C5S] (from B. anthracis) [pubmed|16343540][SW|Localization]
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operons
genes
[gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]-[gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]-[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]
description
[Pubmed|22383849]
view in new tabBiological materials
Mutant
BKE29580 ([gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCTBKK29580 ([gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATATATGATCGTAATTCA, downstream forward: _UP4_TAAATCAATTTTCAGCTCCTReferences
14567704,22773787,16343540,24705700