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kinC [Mon Sep 21 2015 14:57:52 GMT+0200 (CEST)]
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kinC [Mon Sep 21 2015 14:57:52 GMT+0200 (CEST)]

two-component sensor kinase, phosphorylates Spo0F and Spo0A, part of the phosphorelay, governs expression of genes involved in biofilm formation
locus
BSU14490
pI
6.00
mw
48.00 kDa
function
initiation of sporulation
product
two-component sensor kinase
essential
no
synonyms
ssb
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.1|The kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.1|The kinases]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • [SW|Categories] containing this gene/protein

  • [SW|protein modification], [SW|transcription factors and their control], [SW|phosphorelay], [SW|biofilm formation], [SW|membrane proteins], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|Spo0A regulon]

    • Gene

    Phenotypes of a mutant

  • defective in [SW|biofilm formation] [Pubmed|22882210]

    • The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [SW|Spo0F] as part of the [SW|phosphorelay], but also direct phosphorylation of [SW|Spo0A] [Pubmed|19114652]
  • mainly active in the younger, outer regions of a colony (with [protein|search|KinD]) [Pubmed|21097618]
  • phosphorylates [SW|Spo0A] in response to the presence of surfactin [Pubmed|22882210], this has been refuted [Pubmed|25701730]
  • required for initiation of [SW|sliding] together with [protein|search|KinB] [Pubmed|26152584]

    • [SW|Domains]

  • two transmembrane segments
  • [SW|PAS domain]
  • C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue

    • Effectors of protein activity

  • activity is triggered by potassium leakage [Pubmed|19114652], this has been refuted [Pubmed|25701730]
  • activity is triggered by polyisoprenoid lipids formed by [protein|search|YisP] [Pubmed|20713508]
  • activity is stimulated by the interaction with [protein|search|FloT] [Pubmed|26297017]

    • [SW|Localization]

  • cell membrane (Heterogeneous) [Pubmed|16479537]
  • co-localizes with [protein|search|FloT] in discrete foci in the membrane [Pubmed|20713508]
  • the localization of [protein|search|KinC] in membrane microdomains depends on [protein|search|FloA] and [protein|search|FloT] [Pubmed|22882210], this has been refuted [Pubmed|25701730]

    • [SW|Interactions]

  • [protein|search|KinC]-[SW|Spo0A] [Pubmed|19114652]
  • [protein|search|KinC]-[SW|Spo0F]
  • [protein|search|FloT]-[protein|search|KinC] to stimulate [protein|search|KinC] dimerization and activity [Pubmed|26297017]

    • Expression and Regulation

    Operon

  • kinC (according to [http://dbtbs.hgc.jp/COG/prom/kinC.html DBTBS])

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8002614]

    • Regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|8002615]

    • Regulatory mechanism

  • [SW|Spo0A]: transcription activation [Pubmed|8002615]

    • Biological materials

    Mutant

  • 1A632 ( ''kinC''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A632&Search=1A632 BGSC]
  • BAL393 (''kinC''::''spc'')[Pubmed|26152584]

    • References

    Reviews

  • 25934647,25652542

    • Original publications

  • 26152584,19114652,10094672,11069677,16166384,20713508,8002615,16479537,8002614,20946851,20971918,21097618,22882210,23927765,25701730,26297017,26152584