SubtiBank SubtiBank
nasE

nasE

assimilatory nitrite reductase (subunit)
locus
BSU_03290
pI
4.80
mw
11.76 kDa
protein length
106 aa Sequence Blast
gene length
321 bp Sequence Blast
function
utilization of nitrite as nitrogen source
product
assimilatory nitrite reductase (subunit)
essential
no
ec
1.7.1.4
synonyms
nasBD,nirD

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.2|Anaerobic respiration]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.1|Utilization of nitrate/ nitrite]
  • Gene

    Coordinates
    355,412 355,732

    The protein

    Catalyzed reaction/ biological activity

  • 2 H2O + 3 NADP+ + NH4+ --> 5 H+ + 3 NADPH + nitrite (according to UniProt)
  • 2 H2O + 3 NAD+ + NH4+ --> 5 H+ + 3 NADH + nitrite (according to UniProt)
  • [SW|Domains]

  • Rieske domain (aa 8-104) (according to UniProt)
  • [SW|Cofactors]

  • NAD [Pubmed|11289299]
  • FeS cluster [pubmed|29292548]
  • Structure

  • [PDB|5BOK] (from Diaphorobacter sp., 30% identity)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|3F87227DC3371746B3823730B6A7024D348C151E|nasB]-[gene|EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5|nasC]-[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]-[gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]-[gene|159A6D0D45B5F0F055228357FD9A3BF8E5A999E5|nasF]
    description
    [Pubmed|7836289]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7836289], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|10972836], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [PubMed|8799114,9765565], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|NsrR]: repression, [Pubmed|16885456], in [regulon|EC6697D5D945B7E5083AFED9218748763C443278|NsrR regulon]
  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]
  • regulation

  • ''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
  • view in new tab

    genes
    [gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]-[gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]-[gene|159A6D0D45B5F0F055228357FD9A3BF8E5A999E5|nasF]
    description
    [Pubmed|9765565]

    regulation

  • ''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
  • view in new tab

    Biological materials

    Mutant

  • BKE03290 ([gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCATTCCTCCTCAAT, downstream forward: _UP4_TGATCAAAAGACCGGATCAC
  • BKK03290 ([gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCATTCCTCCTCAAT, downstream forward: _UP4_TGATCAAAAGACCGGATCAC
  • References

    Reviews

  • 22103536,11289299
  • Original publications

  • 12823818,7868621,8799114,9765565,10972836,16885456,21091510,24214949,25755103