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catE
catechol 2,3-dioxygenase, detoxification of environmental catechols, protects cells from intoxication by endogenous bacillibactin-derived catechol metabolites under iron-limited conditions
function
detoxification of catechol
product
catechol 2,3-dioxygenase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.7|Detoxification reactions][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]Gene
Coordinates
898,010 898,867
Phenotypes of a mutant
not viable in the presence of catechol [pubmed|30377275]The protein
Catalyzed reaction/ biological activity
catechol + O2 --> (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H+ (according to UniProt)Protein family
[SW|extradiol ring-cleavage dioxygenase family] (according to UniProt)[SW|Domains]
2 [SW|VOC domain]s (aa 9-126, aa 169-285) (according to UniProt)Expression and Regulation
Operons
genes
[gene|B7A0D1B3CAA2733A33BCCDDB6774ABF1A0223473|catD]-[gene|AA75013C05E1A88FC51410F9F1B30EE7D15F34C2|catE]
description
[Pubmed|16872404]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20639328], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|YodB]: repression, [Pubmed|20639328], in [regulon|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|YodB regulon][protein|5B9C4932B2E76D6B8F6BDB640D1ADEE28F488FAD|CatR]: repression, [Pubmed|20639328], in [regulon|5B9C4932B2E76D6B8F6BDB640D1ADEE28F488FAD|CatR regulon][protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|30377275], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]regulation
strong induction by diamide and quinones (such as catechol) ([protein|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|YodB], [protein|5B9C4932B2E76D6B8F6BDB640D1ADEE28F488FAD|CatR]) [Pubmed|16872404,20639328]induced by iron starvation ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [pubmed|30377275]view in new tabBiological materials
Mutant
MGNA-C295 (yfiE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2293 NBRP B. subtilis, Japan]BKE08240 ([gene|AA75013C05E1A88FC51410F9F1B30EE7D15F34C2|catE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTCATGCTGCATGCCTCTT, downstream forward: _UP4_TAACTGCAATCCCCTTGCCGBKK08240 ([gene|AA75013C05E1A88FC51410F9F1B30EE7D15F34C2|catE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTCATGCTGCATGCCTCTT, downstream forward: _UP4_TAACTGCAATCCCCTTGCCGReferences
17407181,16872404,20639328,30377275