pmi

mannose-6-phosphate isomerase

locus: BSU_35790

pI: 4.98

mw: 35.27 kDa

protein length: 316 aa

gene length: 951 bp

function: mannose utilization

product: mannose-6-phosphate isomerase

essential: no

ec: 5.3.1.8

synonyms: yvyI

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.9|Utilization of mannose]

Gene

Coordinates: 3,687,597 3,688,547

The protein

Catalyzed reaction/ biological activity

• D-mannose 6-phosphate --> D-fructose 6-phosphate (according to UniProt)

Protein family

• mannose-6-phosphate isomerase type 1 family (with [protein|E26C70893C5D677C816C814558CC42F90B920087|ManA] and [protein|35225776F6044513D3E77DBBD6A7A8FE976F506A|GmuF], according to UniProt)

Paralogous protein(s)

• [protein|E26C70893C5D677C816C814558CC42F90B920087|ManA], [protein|35225776F6044513D3E77DBBD6A7A8FE976F506A|GmuF]

Structure

• [PDB|1QWR]

Expression and Regulation

Operons

• genes: [gene|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|pmi]
  description: [Pubmed|7934877]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7934877], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• BKE35790 ([gene|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|pmi]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCCGTACCCCTCCCC, downstream forward: _UP4_TAAACTTAATCTCACTTCGA
• BKK35790 ([gene|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|pmi]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCCGTACCCCTCCCC, downstream forward: _UP4_TAAACTTAATCTCACTTCGA

References

• 7934877,23204422