SubtiBank SubtiBank
secA
The second international online conference #Subtillery2021 will be held 14th - 18th June - save the date, for more information see the conference website!

The 21st International Conference on Bacilli has been postponed to 2022 and will take place in Prague.

secA

preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA, motor protein that drives preprotein translocation through the SecY-SecE-SecG channel
locus
BSU_35300
pI
5.34
mw
95.33 kDa
protein length
841 aa Sequence Blast
gene length
2526 bp Sequence Blast
function
protein secretion
product
preprotein translocase subunit (ATPase)
essential
yes
synonyms
div,div-341,ts-341

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    3,628,310 3,630,835

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • terminal phenotype of CRISPRi knockdown mutant is moderate bending and a cessation of growth [Pubmed|27238023]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP ADP + Pi + preprotein translocation
  • required for membrane targeting of [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|DivIVA] [Pubmed|24592260]
  • Protein family

  • SecA family (single member, according to UniProt)
  • [SW|Domains]

  • nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain
  • [SW|Cofactors]

  • Mg
  • Effectors of protein activity

  • anionic phospholipids, preprotein, [protein|5350DC618130E013757EE667B9AFC5F2EC68EC08|SecY], signal peptides (even when added in trans) [Pubmed|19924216]
  • Structure

  • [PDB|1TF5] (open structure), [PDB|2IBM], [PDB|3DL8] (structure of the ([protein|5350DC618130E013757EE667B9AFC5F2EC68EC08|SecY]-[protein|1007EA15FB7AF81A44400287DB68BCC497158ACF|SecE]-[protein|D8851F453E5EF18D36F25834A8CB9C61EAF15E44|SecG])-[protein|AD7CD451B4AE638A719C91780339784A7FF0F248|SecA] complex [Pubmed|18923516]
  • [SW|Localization]

  • cell membrane (according to UniProt)
  • cytoplasm (according to UniProt)
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • Expression and Regulation

    Operons

    genes
    [gene|AD7CD451B4AE638A719C91780339784A7FF0F248|secA]-[gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]
    description
    [Pubmed|9882663]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9882663], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • view in new tab

    Biological materials

    Mutant

  • BEC35300, a CRISPRi knockdown mutant, is available from the [http://bgsc.org/ Bacillus Genetic Stock Center]
  • References

    Reviews

  • 25212246,27890920,27799326
  • Original publications

  • 19933328,19924216,18923516,12873133,12242434,1385592,10816431,19850053,9880811,12218047,17981983,9882663,23484952,20574771,23167435,23794293,23852076,24592260,24786965,11021932,7851746,7894702,10074074,8497195,8440733,7706219,7642557,27336478,27238023,27795329,28041832,27802584,22354575