SubtiBank SubtiBank
secA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

secA

preprotein translocase subunit (ATPase), required for membrane targeting of DivIVA, motor protein that drives preprotein translocation through the SecY-SecE-SecG channel
locus
BSU35300
pI
5.34
mw
95.33 kDa
protein length
841 aa Sequence Blast
gene length
2523 bp Sequence Blast
function
protein secretion
product
preprotein translocase subunit (ATPase)
essential
yes
synonyms
div,div-341,ts-341

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    3,628,310 → 3,630,835

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • terminal phenotype of CRISPRi knockdown mutant is moderate bending and a cessation of growth [Pubmed|27238023]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP → ADP + Pi + preprotein translocation
  • required for membrane targeting of [protein|A8C0C2B7BCA6FFFF3811402D683FB7B99F7120A4|DivIVA] [Pubmed|24592260]
  • Protein family

  • SecA family (according to Swiss-Prot)
  • Paralogous protein(s)

  • none in Bacillus, some species have a paralogous secA gene named secA2 that has an altered substrate range
  • [SW|Domains]

  • nucleotide binding domain, preprotein binding domain, IRA2 domain, scaffold domain, wing domain, IRA1 domain, C-terminal domain
  • [SW|Cofactors]

  • Mg
  • Effectors of protein activity

  • anionic phospholipids, preprotein, [protein|5350DC618130E013757EE667B9AFC5F2EC68EC08|SecY], signal peptides (even when added in trans) [Pubmed|19924216]
  • Structure

  • [PDB|1TF5] (open structure), [PDB|2IBM], [PDB|3DL8] (structure of the ([protein|5350DC618130E013757EE667B9AFC5F2EC68EC08|SecY]-[protein|1007EA15FB7AF81A44400287DB68BCC497158ACF|SecE]-[protein|D8851F453E5EF18D36F25834A8CB9C61EAF15E44|SecG])-[protein|AD7CD451B4AE638A719C91780339784A7FF0F248|SecA] complex [Pubmed|18923516]
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • Expression and Regulation

    Operons

    genes
    [gene|AD7CD451B4AE638A719C91780339784A7FF0F248|secA]-[gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]
    description
    [Pubmed|9882663]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9882663], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • BEC35300, a CRISPRi knockdown mutant, is available from the [http://bgsc.org/ Bacillus Genetic Stock Center]
  • References

    Reviews

  • 25212246,27890920,27799326
  • Original publications

  • 19933328,19924216,18923516,12873133,12242434,1385592,10816431,19850053,9880811,12218047,17981983,9882663,23484952,20574771,23167435,23794293,23852076,24592260,24786965,11021932,7851746,7894702,10074074,8497195,8440733,7706219,7642557,27336478,27238023,27795329,28041832,27802584,22354575