ppaC

inorganic pyrophosphatase

locus: BSU_40550

pI: 4.51

mw: 33.83 kDa

protein length: 309 aa

gene length: 930 bp

function: recovery of phosphate ions from pyrophosphate

product: inorganic pyrophosphatase

essential: yes

ec: 3.6.1.1

synonyms: yybQ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 4,168,204 4,169,133

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• diphosphate + H₂O --> H⁺ + 2 phosphate (according to UniProt)

Protein family

• PPase class C family (single member, according to UniProt)

[SW|Domains]

• [SW|DHH-DHHA1 domain]

Modification

• S-cysteinylation after diamide stress (C158) [Pubmed|17611193]
• Cys158 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|21749987] [Pubmed|21749987]

[SW|Cofactors]

• Mn²⁺ [pubmed|17095506]

Structure

• [PDB|2HAW] (complex with substrate analogue p-nitrophosphate), [PDB|1WPM] [pubmed|17095506]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|AE44787B3EB8EF5811B5C447BE2012EC36547B0A|ppaC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B837 (yybQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1836 NBRP B. subtilis, Japan]

References

• 22938038,21749987,15533045,17611193,9845334,9782505,11342544,9633597,21749987,15378759,22383849,25733611,17095506