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amhX

amidohydrolase

locus: BSU_03010

pI: 6.12

mw: 42.37 kDa

protein length: 383 aa

gene length: 1152 bp

function: unknown

product: amidohydrolase

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 324,038 325,189

The protein

Protein family

• [SW|peptidase M20 family] (according to UniProt)

Paralogous protein(s)

• [protein|C99AA3FDBB561BCED60915078DC746690C071753|SndB]

Structure

• [PDB|1YSJ] (from ''Bacillus subtilis'' (32%) )

Expression and Regulation

Operons

• genes: [gene|AE824A62089631F129C3AA469B6ACA71DC13D2F8|amhX]
  description: [Pubmed|8768514]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8768514], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|24843172], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

Biological materials

Mutant

• BKE03010 ([gene|AE824A62089631F129C3AA469B6ACA71DC13D2F8|amhX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGAAATACCTCCT, downstream forward: _UP4_TAACGCAAAAAAAGCAGCCT
• BKK03010 ([gene|AE824A62089631F129C3AA469B6ACA71DC13D2F8|amhX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGAAATACCTCCT, downstream forward: _UP4_TAACGCAAAAAAAGCAGCCT

References

• 8768514,24843172