SubtiBank SubtiBank
yhcV
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yhcV

forespore-specific sporulation protein
locus
BSU_09230
pI
5.01
mw
14.77 kDa
protein length
140 aa Sequence Blast
gene length
423 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • Gene

    Coordinates
    997,175 → 997,597

    The protein

    Paralogous protein(s)

  • [protein|8529D83560E934709B20CCF0B72251A74EC272BB|YlbB]:
  • [SW|Domains]

  • 2 [SW|CBS domain]s (aa 8-64, aa 72-127) (according to UniProt)
  • Structure

  • [PDB|4FRY] (protein from ''Burkholderia ambifaria'', 30% identity) [Pubmed|23382856]
  • Additional information

  • The gene is annotated in KEGG as an ortholog of IMP dehydrogenase EC 1.1.1.205. No EC annotation is available in Swiss-Prot/MetaSwiss-Prot/MetaCyc.literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]
  • Expression and Regulation

    Operons

    genes
    [gene|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|yhcV]
    description
    [Pubmed|12480901]

    sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190,12480901,16497325], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]
  • regulation

  • expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|15699190,12480901,16497325]
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    additional information

  • the mRNA belongs to the 46 most abundant mRNA species in dormant spores [pubmed|30782632]
  • Biological materials

    Mutant

  • MGNA-B474 (yhcV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1473 NBRP B. subtilis, Japan]
  • BKE09230 (Δ[gene|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|yhcV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCAGCACCCCTTTCA, downstream forward: _UP4_TAATAAGAAAGAGCTTGCAG
  • BKK09230 (Δ[gene|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|yhcV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCAGCACCCCTTTCA, downstream forward: _UP4_TAATAAGAAAGAGCTTGCAG
  • Expression vectors

  • pGP2930 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • References

  • 15699190,12480901,16497325,23382856,30782632