SubtiBank SubtiBank
bglC
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bglC

endo-1,4-beta-glucanase
locus
BSU_18130
pI
8.62
mw
55.12 kDa
protein length
499 aa Sequence Blast
gene length
1500 bp Sequence Blast
function
beta-1,4-glucan degradation
product
endo-1,4-beta-glucanase)
essential
no
ec
3.2.1.4
synonyms
eglS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    1,940,625 → 1,942,124

    The protein

    Catalyzed reaction/ biological activity

  • Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans (according to UniProt)

    • Protein family

  • glycosyl hydrolase 5 (cellulase A) family (single member, according to UniProt)

    • [SW|Domains]

  • CBM3 domain (aa 350-499) (according to UniProt)

    • Structure

  • [PDB|3PZT] [Pubmed|21880019]

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|B0528C4772B2357D10AEEA3C63080C5176B27E7B|bglC]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3106328], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • 1A751 (no resistance), [Pubmed|7704256], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A751&Search=1A751 BGSC]
  • 1A752 (no resistance), [Pubmed|7704256], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A752&Search=1A752 BGSC]
  • 1A976 (no resistance), [Pubmed|21255377], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A976&Search=1A976 BGSC]
  • BKE18130 (Δ[gene|B0528C4772B2357D10AEEA3C63080C5176B27E7B|bglC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTGATCTTTTCCTCC, downstream forward: _UP4_TAGTTAAGCTTTTTTTTGGC
  • BKK18130 (Δ[gene|B0528C4772B2357D10AEEA3C63080C5176B27E7B|bglC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTGATCTTTTCCTCC, downstream forward: _UP4_TAGTTAAGCTTTTTTTTGGC

    • References

  • 18957862,3024130,21880019,3106328,23543074,27334041