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SEDS family peptidoglycan glycosyltransferase, part of the [protein|search|Rod complex] for lateral [SW|cell wall synthesis] and control of cell diameter
function
control of cell shape and elongation
product
SEDS family peptidoglycan glycosyltransferase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
3,912,332 → 3,913,513
Phenotypes of a mutant
essential [Pubmed|12682299]CRISPRi knockdown mutant has a terminal phenotype of severe lysis [Pubmed|27238023]The protein
Catalyzed reaction/ biological activity
principal peptidoglycan glycosyltransferase of the [SW|elongasome] [Pubmed|27525505][GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H+ (according to UniProt)Protein family
[SW|SEDS proteins] (shape, elongation, division, and sporulation) [Pubmed|27525505]SEDS family (with [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|FtsW] and [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|SpoVE], according to UniProt)Paralogous protein(s)
[protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|SpoVE], [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|FtsW] Structure
[PDB|6BAR] (from Thermus thermophilus, 26% identity) [pubmed|29590088][SW|Localization]
integral membrane protein [Pubmed|21636744]forms circumferentially moving patches [Pubmed|21636744]localization depends on [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] [SW|26679002]Expression and Regulation
Operons
genes
[gene|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA]
description
[Pubmed|9353933]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]view in new tabBiological materials
Mutant
xylose-inducible CRISPRi knockdown mutant BEC38120 is available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Daniel_R._Zeigler BGSC]labs
[SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage][SW|David Rudner], Harvard Medical School, MA, USA [http://rudnerlab.med.harvard.edu/ homepage]References
Reviews
Original Publications
27525505, 27238023, 9622350,21636744,21636745,17981970,28085152,29590088,31086310,32152588,32973704