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fadE
function
fatty acid degradation
product
acyl-CoA dehydrogenase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
3,367,040 → 3,368,824
The protein
Catalyzed reaction/ biological activity
A + 2,3-saturated acyl-CoA --> 2,3-dehydroacyl-CoA + AH2 (according to UniProt)Protein family
[SW|Acyl-CoA dehydrogenase family] (according to UniProt)Paralogous protein(s)
[protein|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|YngJ], [protein|7D89BBB52403BF99416250688EFA90C2BE8EB591|AcdA][protein|F25CDDEBAFC72036664323619F5197D45E86BE9A|MmgC]: (40.2%) [SW|Cofactors]
FAD (according to UniProt)Structure
[PDB|2Z1Q] (from ''Thermus thermophilus hb8'', 52% identity, 68% similarity)[SW|Localization]
cell membrane [Pubmed|18763711]Biological materials
Mutant
MGNA-A602 (yusJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/602 NBRP B. subtilis, Japan]BKE32820 (''[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]BKE32820 (Δ[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTTTTCCCCCTTTA, downstream forward: _UP4_TGATTGGCCATGAGCTCCGCBKK32820 (Δ[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTTTTCCCCCTTTA, downstream forward: _UP4_TGATTGGCCATGAGCTCCGCReferences
17189250,12817086,18763711,23033921,21398533