SubtiBank SubtiBank
dfrA
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dfrA

dihydrofolate reductase
locus
BSU_21810
pI
5.22
mw
19.03 kDa
protein length
168 aa Sequence Blast
gene length
507 bp Sequence Blast
function
biosynthesis of folate
product
dihydrofolate reductase
essential
yes
ec
1.5.1.3
synonyms
tmp

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.5|Biosynthesis of folate]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,296,603 → 2,297,109

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • (6S)-5,6,7,8-tetrahydrofolate + NADP+ --> 7,8-dihydrofolate + H+ + NADPH (according to UniProt)
  • Protein family

  • dihydrofolate reductase family (single member, according to UniProt)
  • [SW|Domains]

  • DHFR domain (aa 1-159) (according to UniProt)
  • Structure

  • [PDB|1ZDR] (Geobacillus stearothermophilus)
  • Expression and Regulation

    Operons

    genes
    [gene|8B515C789439354F870023E69077D2C1D709E046|ypjP]-[gene|2CB3BAF17F59A858D249619A436EF660CAA58DD5|ypjQ]-[gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]-[gene|9D1DAA5E69DD12FDE0FB33EA0863E0CB214120EC|ypkP]
    description
    [Pubmed|22383849]
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    genes
    [gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]
    description
    [Pubmed|2840350]

    regulatory mechanism

  • [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN]: activation, weak, in [regulon|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN regulon]
  • regulation

  • weak induction in the presence of glucose ([protein|search|CcpN]) [Pubmed|19732150]
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    References

  • 2840350,6436144,3918568,24158440