SubtiBank SubtiBank
hprT

hprT

trigger enzyme, hypoxanthine phosphoribosyltransferase and part of a transcription activator
locus
BSU00680
pI
4.60
mw
20.10 kDa
protein length
180 aa Sequence Blast
gene length
540 bp Sequence Blast
function
purine salvage and interconversion, control of ftsH expression
product
hypoxanthine phosphoribosyltransferase
essential
no
ec
2.4.2.8
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.3|Purine salvage and interconversion]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    76,344 → 76,886

    Phenotypes of a mutant

  • essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
  • The protein

    Catalyzed reaction/ biological activity

  • IMP diphosphate = hypoxanthine 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)
  • Protein family

  • purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
  • Effectors of protein activity

  • inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´[Pubmed|22981860]
  • Structure

  • [PDB|1J7J] (from ''Salmonella typhimurium'', 51% identity, 73% similarity)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
    description
    [Pubmed|18179421]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7608085], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|TilS]: activation, with [protein|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|HprT] [Pubmed|24001521], in [regulon|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|TilS regulon]
  • [protein|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|HprT]: activation, with [protein|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|TilS] [Pubmed|24001521], in [regulon|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|HprT regulon]
  • regulation

  • induced by heat shock (class III)
  • additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
  • view in new tab

    genes
    [gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]-[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]-[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]-[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|16497325]
  • view in new tab

    References

    Reviews

  • 28031352
  • Original publications

  • 18179421,22981860,24001521,6408059,28189581