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narG
nitrate reductase (alpha subunit)
function
nitrate respiration, nitrogen assimilation
product
nitrate reductase (alpha subunit)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.2|Anaerobic respiration][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
3,826,259 → 3,829,945
The protein
Catalyzed reaction/ biological activity
quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)Protein family
[SW|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)[SW|Domains]
[SW|4Fe-4S Mo/W bis-MGD-type domain] (aa 47-111) (according to UniProt)[SW|Cofactors]
Fe-S cluster [Pubmed|11289299]MoCo [Pubmed|11289299]Structure
[PDB|1Q16] (the [protein|B992698A12EE9D17DCEF075F82396D5456D35A20|NarG]-[protein|6181C785427277C097B81D67A93213AEAF701FF4|NarH]-[protein|20060F4C1939FB9A10CB279745D75D0A16627EC6|NarI] complex of E. coli, 52% identity) [pubmed|12910261][SW|Localization]
cell membrane [Pubmed|23880299]Expression and Regulation
Operons
genes
[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]-[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]
description
[Pubmed|8846791,16428414]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8846791,16428414], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: activation, [Pubmed|8846791,16428414], in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]regulation
expressed under anaerobic conditions ([protein|search|Fnr]) [Pubmed|8846791,16428414]view in new tabBiological materials
Mutant
BKE37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGABKK37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGAReferences
Reviews
Original publications
9352926,23880299,8846791,16428414,12910261