SubtiBank SubtiBank
narG
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narG

nitrate reductase (alpha subunit)
locus
BSU_37280
pI
6.06
mw
138.86 kDa
protein length
1228 aa Sequence Blast
gene length
3687 bp Sequence Blast
function
nitrate respiration, nitrogen assimilation
product
nitrate reductase (alpha subunit)
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.2|Anaerobic respiration]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    3,826,259 → 3,829,945

    The protein

    Catalyzed reaction/ biological activity

  • quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
  • Protein family

  • [SW|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)
  • [SW|Domains]

  • [SW|4Fe-4S Mo/W bis-MGD-type domain] (aa 47-111) (according to UniProt)
  • [SW|Cofactors]

  • Fe-S cluster [Pubmed|11289299]
  • MoCo [Pubmed|11289299]
  • Structure

  • [PDB|1Q16] (the [protein|B992698A12EE9D17DCEF075F82396D5456D35A20|NarG]-[protein|6181C785427277C097B81D67A93213AEAF701FF4|NarH]-[protein|20060F4C1939FB9A10CB279745D75D0A16627EC6|NarI] complex of E. coli, 52% identity) [pubmed|12910261]
  • [SW|Localization]

  • cell membrane [Pubmed|23880299]
  • Expression and Regulation

    Operons

    genes
    [gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]-[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]
    description
    [Pubmed|8846791,16428414]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8846791,16428414], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: activation, [Pubmed|8846791,16428414], in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]
  • regulation

  • expressed under anaerobic conditions ([protein|search|Fnr]) [Pubmed|8846791,16428414]
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    Biological materials

    Mutant

  • BKE37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
  • BKK37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
  • References

    Reviews

  • 11289299
  • Original publications

  • 9352926,23880299,8846791,16428414,12910261