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rnc
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rnc

RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
locus
BSU_15930
pI
8.08
mw
28.28 kDa
protein length
249 aa Sequence Blast
gene length
750 bp Sequence Blast
function
processing and degradation of RNA molecules
product
endoribonuclease III
essential
yes
synonyms
rncS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    1,665,710 → 1,666,459

    Phenotypes of a mutant

  • essential [Pubmed|23300471,12682299,11123676]
  • the ''[gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]'' gene can be deleted in strains cured of the [SW|Skin element] and [SW|SP-beta prophage] (or upon deletion of the toxin genes ''[gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]'' and ''[gene|961DB64225CEF84D80982ADBDC9BBBCDF5049132|yonT]'') [Pubmed|23300471]

    • additional information

  • [SW|RNase III targets| RNAs that are targeted by RNase III] [pubmed|26883633]

    • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
  • cleaves 16S rRNA 2 nts downstream of the 3' end [PubMed|26883633]
  • cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]'')
  • degradation of ''[gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]'' mRNA-[protein|search|FFCE5AD9E04E6D5F7970E89781C545E7DBDF45F0] RNA duplex (encoded on the [SW|Skin element]) [Pubmed|23300471]
  • degradation of ''[gene|961DB64225CEF84D80982ADBDC9BBBCDF5049132|yonT]'' mRNA-''[gene|8832F99BF927D5A4D6CB265DB24D3AE46434A5CD|SR6]'' RNA duplex (encoded on the [[SP-beta prophage]]) [Pubmed|23300471]
  • degradation of the ''[gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]''-''[gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI]'' and ''[gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]-[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]'' RNA duplexes [Pubmed|25643072]

    • Protein family

  • ribonuclease III family (single member, according to UniProt)

    • [SW|Domains]

  • RNase III domain (aa 20-149) (according to UniProt)
  • DRBM domain (aa 175-222) (according to UniProt)

    • Structure

  • [PDB|1O0W] (from ''Thermotoga maritima'', 41% identity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]-[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]-[gene|D519765BF5261E48512CE86245BD7B733F188D4A|ftsY]
    description
    [Pubmed|11021934]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11021934], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

    • regulation

  • expressed during vegetative growth and during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|11021934]
    • view in new tab

    additional information

  • the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

    • Biological materials

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab, [pubmed|19193632]

    • labs

  • [SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
  • [SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]

    • References

    Reviews

  • 17194582,16179796,8936814,16855311,23300472,25292357,25808661,25878039,29651979,31464530

    • Original publications

  • 9393702,11123676,7527387,9677377,17576666,11021934,6409421,22123973,22412379,25643072,23300471,26940229,26883633