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pdaB
polysaccharide deacetylase
function
spore cortex formation
product
polysaccharide deacetylase
Genomic Context
categories
[category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]Gene
Coordinates
159,779 → 160,543
Phenotypes of a mutant
''[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB] [gene|BB0443D95581DCA66EDA390842945F105A71A371|ytrI]'' and ''[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB] [gene|D5B9ACB1E447A893C99A4ACE1DA95EB38BCF971B|ytrH]'' mutants are deficient in sporulationinactivation of ''[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB]'' reduces sporulation efficiency to 5% that of wild type cells [Pubmed|26735940]The protein
Protein family
[SW|polysaccharide deacetylase family] (according to UniProt)[SW|Domains]
[SW|NodB homology domain] (aa 57-237) (according to UniProt)Structure
[PDB|4M1B] (from B. anthracis, 54% identity) [pubmed|24637747][SW|Localization]
forespore (according to Swiss-Prot)Expression and Regulation
Operons
genes
[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB]
description
[Pubmed|12662922]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|12662922], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]regulation
expressed during sporulation in the mother cell [Pubmed|12662922]additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]view in new tabBiological materials
Mutant
MGNA-B947 (ybaN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1946 NBRP B. subtilis, Japan]BKE01570 (Δ[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGATGATGCCTCCTTAT, downstream forward: _UP4_GCCAAATCCGCAGAGGTAAABKK01570 (Δ[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGATGATGCCTCCTTAT, downstream forward: _UP4_GCCAAATCCGCAGAGGTAAAReferences
12662922,12884008,15547282,15598884,26735940,24637747