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response regulator aspartate phosphatase, dephosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F]-P
function
control of the [SW|phosphorelay]
product
response regulator aspartate phosphatase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.2|Response regulator aspartate phosphatase]Gene
Coordinates
304,430 → 305,551
The protein
Catalyzed reaction/ biological activity
dephosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F]-P [Pubmed|21346797]Protein family
[SW|RAP family] (according to UniProt)[SW|Domains]
RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain (five [SW|TPR repeat|tetratrichopeptide repeats]) that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide [protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|PhrC], the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. [Pubmed|23526881]Effectors of protein activity
the interaction with [protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|PhrC] inactivates [protein|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|RapJ] [Pubmed|23526881]Structure
[PDB|4GYO] (complex [protein|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|RapJ]-[protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|PhrC]) [Pubmed|23526881]Expression and Regulation
Operons
genes
[gene|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]
description
[pubmed|22383849]
view in new tabBiological materials
Mutant
BKE02820 (Δ[gene|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGTCTGCCTCCTTCCT, downstream forward: _UP4_TAGGAAATGGCAGAGAACTABKK02820 (Δ[gene|BC28167FE175BFD7DBF1B07C54D3512184B95B8F|rapJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGTCTGCCTCCTTCCT, downstream forward: _UP4_TAGGAAATGGCAGAGAACTAReferences