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pupG
purine nucleoside phosphorylase
function
purine salvage and interconversion
product
purine nucleoside phosphorylase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.3|Purine salvage and interconversion][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]Gene
Coordinates
2,446,418 → 2,447,233
The protein
Catalyzed reaction/ biological activity
purine 2'-deoxy-D-ribonucleoside + phosphate --> 2-deoxy-α-D-ribose 1-phosphate + purine nucleobase (according to UniProt)Protein family
PNP/MTAP phosphorylase family (single member, according to UniProt)Modification
phosphorylation on Ser-28 [Pubmed|17218307]Structure
[PDB|3LA8] (from Streptococcus mutans, 57% identity)[SW|Localization]
secreted (according to Swiss-Prot)Expression and Regulation
Operons
genes
[gene|9D07E43BF1FFB13CCEAC2B7AAA4EBDFB1079DD74|drm]-[gene|BC6FA7677AAE6BA24530D125007A18056D75DADF|pupG]
description
[Pubmed|10537218]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10537218], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|22900538], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]regulation
induced in the presence of nucleosides (deoxyribose 5-phosphate and ribose 5-phosphate act as molecular inducers) [Pubmed|10537218]view in new tabBiological materials
Mutant
BKE23490 (Δ[gene|BC6FA7677AAE6BA24530D125007A18056D75DADF|pupG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTGTCCTTCAAGAAACAGT, downstream forward: _UP4_TAAATATGAATCAATGCAGGBKK23490 (Δ[gene|BC6FA7677AAE6BA24530D125007A18056D75DADF|pupG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTGTCCTTCAAGAAACAGT, downstream forward: _UP4_TAAATATGAATCAATGCAGGReferences
10537218,22900538,17218307