SubtiBank SubtiBank
hutI
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hutI

imidazolone-5-propionate hydrolase
locus
BSU_39370
pI
5.22
mw
45.40 kDa
protein length
421 aa Sequence Blast
gene length
1266 bp Sequence Blast
function
histidine utilization
product
imidazolone-5-propionate hydrolase
essential
no
ec
3.5.2.7
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.5|Utilization of histidine]
  • Gene

    Coordinates
    4,045,245 → 4,046,510

    The protein

    Catalyzed reaction/ biological activity

  • 4-imidazolone-5-propanoate + H2O --> N-formimidoyl-L-glutamate (according to UniProt)
  • Protein family

  • [SW|Metallo-dependent hydrolases superfamily] (according to UniProt)
  • Structure

  • [PDB|2BB0] [Pubmed|16990261]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP]-[gene|DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888|hutH]-[gene|ED6002BB56B96F9A2B4F80CA050518B266000E55|hutU]-[gene|BD3382760B704972A0E67ECEDA4B29AA4ECD8164|hutI]-[gene|FBB15E07B7134C57208EA82D5339708385DBC5FB|hutG]-[gene|423AEC9D260BE53AD22851D999244B89BEB3E84D|hutM]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8071225], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|8071225], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|8682780], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|HutP]: antitermination, at a protein-dependent [SW|RNA switch] located between [gene|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|hutP] and [gene|DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888|hutH] [Pubmed|8071225], in [regulon|BCA2700F27CA7A0C3FC08AAEBE615A783A42F408|HutP regulon]
  • regulation

  • induced by histidine ([protein|search|HutP]) [Pubmed|8071225]
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    Biological materials

    Mutant

  • BKE39370 (Δ[gene|BD3382760B704972A0E67ECEDA4B29AA4ECD8164|hutI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCTTTGGCATGTCATCAC, downstream forward: _UP4_GTTGTCAACAGGGAGGGAGC
  • BKK39370 (Δ[gene|BD3382760B704972A0E67ECEDA4B29AA4ECD8164|hutI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGCTTTGGCATGTCATCAC, downstream forward: _UP4_GTTGTCAACAGGGAGGGAGC
  • References

    Reviews

  • 22933560
  • Original publications

  • 10746760,8071225,8682780,10217782,16990261,27711543,18442260