ywpB

ß-hydroxyacyl-(acyl carrier protein) dehydratase

locus: BSU_36370

pI: 9.36

mw: 14.63 kDa

protein length: 141 aa

gene length: 426 bp

function: fatty acid biosynthesis

product: β-hydroxyacyl-ACP dehydratase

essential: yes

ec: 4.2.1.59

synonyms: fabZ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,743,732 → 3,744,157

Phenotypes of a mutant

• essential [Pubmed|23109554]

The protein

Catalyzed reaction/ biological activity

• (3R)-hydroxyacyl-[ACP] --> (2E)-enoyl-[ACP] + H₂O (according to UniProt)

Protein family

• thioester dehydratase family (with [protein|65506215AE58BF53C57599E566E4F78514DB45AA|YcsD], according to UniProt)

Paralogous protein(s)

• [protein|65506215AE58BF53C57599E566E4F78514DB45AA|YcsD]

Structure

• [PDB|3D6X] (from ''Campylobacter jejuni'', 45% identity, 67% similarity) [Pubmed|19280690]

Expression and Regulation

Operons

• genes: [gene|BDA23FA513C240B07DC109BB62A7A5EB96B20965|ywpB]-[gene|7F7BC285A1D02B031575BA7E980293CD2962C84A|mscL]
  description: [Pubmed|22383849]

  regulation

  • expressed in logarithmic phase [Pubmed|19252899]

Biological materials

Mutant

• MGNA-B265 (ywpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1264 NBRP B. subtilis, Japan]

References

• 17919287,19280690