SubtiBank SubtiBank
hisF
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hisF

imidazole glycerol phosphate synthase (synthase subunit)
locus
BSU_34870
pI
5.24
mw
27.14 kDa
protein length
252 aa Sequence Blast
gene length
759 bp Sequence Blast
function
biosynthesis of histidine
product
imidazole glycerol phosphate synthase (synthase subunit)
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.14|Biosynthesis/ acquisition of histidine]
  • Gene

    Coordinates
    3,583,562 → 3,584,320

    The protein

    Catalyzed reaction/ biological activity

  • 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + L-glutamine --> 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H+ + L-glutamate (according to UniProt)
  • Protein family

  • HisA/HisF family (with [protein|148F0C7983250F50676D234DB713E532AA55DDBF|HisA], according to UniProt)
  • Paralogous protein(s)

  • [protein|148F0C7983250F50676D234DB713E532AA55DDBF|HisA]
  • Structure

  • [PDB|1KA9] (the [protein|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|HisF]-[protein|DEB3122A3EA36545759FAA6F86F71F971CA5F011|HisH] complex from ''Thermus thermophilus'', 59% identity) [Pubmed|12417026]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|E8FD92F48BE16F87D7481262F4E49003A78DE78F|hisZ]-[gene|183C8B4F28E601D670B3D997D333AF2B5C4A4759|hisG]-[gene|03274B80B2DFE0A2939981B0E0CB8C34A9B0B102|hisD]-[gene|88ED20DFEBD5D7B985F99D4494142FBA699A1E91|hisB]-[gene|DEB3122A3EA36545759FAA6F86F71F971CA5F011|hisH]-[gene|148F0C7983250F50676D234DB713E532AA55DDBF|hisA]-[gene|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|hisF]-[gene|5C84CA2A9E22173E144F519389F545F107A267D9|hisI]
    description
    [Pubmed|12107147]

    regulatory mechanism

  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR]: regulation, [pubmed|30355672], in [regulon|F4097349A563503468A2A14F062AEAC532C7917A|YlxR regulon]
  • regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
  • expression depends on functional [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]
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    Biological materials

    Mutant

  • BKE34870 (Δ[gene|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|hisF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCATGGGATAATCCGTTTTG, downstream forward: _UP4_AAAGAGTACGGGGTGAATGT
  • BKK34870 (Δ[gene|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|hisF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCATGGGATAATCCGTTTTG, downstream forward: _UP4_AAAGAGTACGGGGTGAATGT
  • References

    Reviews

  • 12859215
  • Original publications

  • 12107147,15378759,12417026,27766092