SubtiBank SubtiBank
ycgT

ycgT

NADPH:ferredoxin oxidoreductase
locus
BSU_03270
pI
5.61
mw
36.81 kDa
protein length
336 aa Sequence Blast
gene length
1011 bp Sequence Blast
function
unknown
product
NADPH:ferredoxin oxidoreductase
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]
  • Gene

    Coordinates
    352,858 → 353,868

    The protein

    Catalyzed reaction/ biological activity

  • H+ + NADP+ + 2 reduced [2Fe-2S]-[ferredoxin] --> NADPH + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
  • Protein family

  • ferredoxin--NADP reductase type 2 family (with [protein|BAA74A6971CED9FB55540F1BC602CDA287D83E74|YumC], according to UniProt)
  • Paralogous protein(s)

  • [protein|BAA74A6971CED9FB55540F1BC602CDA287D83E74|YumC]
  • [SW|Cofactors]

  • FAD [Pubmed|25826316]
  • Structure

  • [PDB|5XHU]
  • Additional information

  • The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxin
  • reductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]
  • Expression and Regulation

    Operons

    genes
    [gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [Pubmed|16672620], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]
  • regulation

  • induced under conditions of iron starvation ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|16672620]
  • additional information

  • the mRNA is very stable (half-life > 15 min) [Pubmed|12884008]
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    Biological materials

    Mutant

  • MGNA-C052 (ycgT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2050 NBRP B. subtilis, Japan]
  • GP1852 (Δ[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]::cat), available in [SW|Jörg Stülke]'s lab
  • BKE03270 (Δ[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT, downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
  • BKK03270 (Δ[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT, downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
  • References

  • 12884008,16672620,25826316,20878669